CAJ | 학술논문

研究建立了肉和肉制品中大肠杆菌的检验及计数方法，分别对金黄色葡萄球菌等9种阴性菌株和1种阳性菌株（大肠埃希氏菌（ATCC25922））进行了试验。结果发现，9种阴性菌株在纤维素乙酸酯膜上都没有菌落生长，而阳性菌株大肠埃希氏菌（ATCC25922）在纤维素乙酸酯膜上的菌落吲哚反应为阳性，试验结果没有出现假阳性或假阴性情况，方法准确性较高，特异性强。
연구건립료육화육제품중대장간균적검험급계수방법，분별대금황색포도구균등9충음성균주화1충양성균주（대장애희씨균（ATCC25922））진행료시험。결과발현，9충음성균주재섬유소을산지막상도몰유균락생장，이양성균주대장애희씨균（ATCC25922）재섬유소을산지막상적균락신타반응위양성，시험결과몰유출현가양성혹가음성정황，방법준학성교고，특이성강。
Meat and meat products are the main food in people's daily life due to the high quality nutrients of them. However, they are also liable to be contaminated by some kinds of harmful microbe, especially Escherichia coli. In this work, the examination and enumeration method of Escherichia coli for meat and meat products was established, and then 9 negative bacterial strains, such as staphylococcus aureus, and 1 positive bacterial strain [Escherichia coli (ATCC25922)] were examined. The results found that there was no bacterial colony growing on the cellulose acetate membrane for 9 negative bacterial strains, while the indole reaction for Escherichia coli is positive. More importantly, there were no false-positive and false-negative situations in the experimental results. This method not only has a high accuracy, but also has a strong specificity.