CAJ | 학술논문

在单因素试验的基础上，利用响应曲面法对纤维素酶辅助乙醇浸提黑米皮花色苷提取工艺参数进行优化研究。选择酶解温度、酶用量和酶解时间为自变量，花色苷提取量为响应值，采用Box-Behnken设计方法，研究各自变量及其交互作用对花色苷提取率的影响。利用Design Expert 7.0软件对数据进行回归分析，得到二次多项式回归方程的预测模型。结果表明，纤维素酶辅助提取黑米花色苷的优化工艺条件为：加酶量为2.0%，酶解温度为38.7℃，酶解时间为128.8 min，料液比为1∶10，浸提时间为40 min，浸提温度为50℃，乙醇浸提液浓度为80%，在此条件下，黑米皮中花色苷的提取量理论值达21.9 mg/g。
재단인소시험적기출상，이용향응곡면법대섬유소매보조을순침제흑미피화색감제취공예삼수진행우화연구。선택매해온도、매용량화매해시간위자변량，화색감제취량위향응치，채용Box-Behnken설계방법，연구각자변량급기교호작용대화색감제취솔적영향。이용Design Expert 7.0연건대수거진행회귀분석，득도이차다항식회귀방정적예측모형。결과표명，섬유소매보조제취흑미화색감적우화공예조건위：가매량위2.0%，매해온도위38.7℃，매해시간위128.8 min，료액비위1∶10，침제시간위40 min，침제온도위50℃，을순침제액농도위80%，재차조건하，흑미피중화색감적제취량이론치체21.9 mg/g。
In this study, we used the response surface method on the base of single factor test, to optimize the extract process of cellulase-assisted extraction of anthocyanins from black rice. The enzymes dosage, enzymolysis time and temperature are studied with Box-Behnken design, the interactive effect on the influence of anthocyanins extraction was also predictive model of polynomial quadratic equation is by Design Expert 7.0 software. We chose the best solution as follows:2.0%adding amount of enzyme, enzymolysis time of 128.8 min at 38.7℃, solid-liquid ration for 1∶10, extracted for 40 min at 50℃in 80%ethanol solution. Under the optimized condition, the predicted extraction rate of the anthocyanins from the black rice pericarp is 21.9 mg/g.