CAJ | 학술논문

目的：探讨B7-H4在胃癌组织中表达及与患者临床病理特征、预后的相关性和可能的临床意义。方法应用反转录聚合酶链反应（RT-PCR）技术及免疫组织化学染色检测B7-H4在胃癌组织中的表达，统计分析 B7-H4表达与临床病理参数的关系。结果 B7-H4在胃癌中高表达（71/100），其表达强度与浸润深度、淋巴结转移及病理分期相关。生存期≤5年的患者B7-H4阳性表达率高于生存期＞5年的患者，差异有统计学意义（P＜0.05），此外，B7-H4阳性患者的预后较B7-H4阴性患者差，差异有统计学意义（P＜0.05）。结论 B7-H4蛋白表达与胃癌患者预后密切相关，可能成为胃癌的一个肿瘤标志物。
목적：탐토B7-H4재위암조직중표체급여환자림상병리특정、예후적상관성화가능적림상의의。방법응용반전록취합매련반응（RT-PCR）기술급면역조직화학염색검측B7-H4재위암조직중적표체，통계분석 B7-H4표체여림상병리삼수적관계。결과 B7-H4재위암중고표체（71/100），기표체강도여침윤심도、림파결전이급병리분기상관。생존기≤5년적환자B7-H4양성표체솔고우생존기＞5년적환자，차이유통계학의의（P＜0.05），차외，B7-H4양성환자적예후교B7-H4음성환자차，차이유통계학의의（P＜0.05）。결론 B7-H4단백표체여위암환자예후밀절상관，가능성위위암적일개종류표지물。
Objective To study the expression of B7-H4 in gastric carcinoma tissues and investigate the relationship between the expression of B7-H4 and the clinic-pathological features and the prognosis. Methods The expression of B7-H4 mRNA and B7-H4 protein were detected by RT-PCR and immunohistochemistry in gastric carcinoma tissues, the relationship between the expression of B7-H4 and the clinic-pathological features were analysed. Results The expression of B7-H4 mRNA was in both gastric carcinoma tissues and nearly normal tissues, but the expression of B7-H4 in gastric carcinoma tissues was higher than in nearly normal tissues;High expression of B7-H4 in gastric carcinoma tissues, the positive rate of B7-H4 was 71%, the expression of B7-H4 protein was positively correlated to the invasion depth, lymph node metastasis and pathological staging, the positive expression rate of B7-H4 of the patient with less than 5-year-life was obviously higher that that of the positive with more than 5-year-life, the difference had statistic significance(P<0.05). Conclusion The expression of B7-H4 protein may be closely related to metastasis and prognosis, and B7-H4 also may be a vital marker in gastric carcinoma tissues.