CAJ | 학술논문

为优选紫花苜蓿叶蛋白的提取工艺条件，对不同品系的苜蓿叶蛋白进行差异性分析，本试验利用单因素极差分析，以280nm紫外光测测定并计算提取率，L9(33)正交试验设计确定最佳提取工艺。结果表明，通过单因素试验确定的各因素影响能力大小为：pH>浸泡时间>料液比>温度，正交试验最佳组合为A2B1C1。结果中三得利（兰州）对照和MA-7中叶蛋白的含量相对较高，分别为21.91%和19.42%。
위우선자화목숙협단백적제취공예조건，대불동품계적목숙협단백진행차이성분석，본시험이용단인소겁차분석，이280nm자외광측측정병계산제취솔，L9(33)정교시험설계학정최가제취공예。결과표명，통과단인소시험학정적각인소영향능력대소위：pH>침포시간>료액비>온도，정교시험최가조합위A2B1C1。결과중삼득리（란주）대조화MA-7중협단백적함량상대교고，분별위21.91%화19.42%。
To select the alfalfa leaf protein extraction process conditions and analyze difference for different strains of alfalfa leaf protein, the single factor analysis of range method was used in this study, leaf protein extraction rate were measured in 280nm by uv spectrophotometry, the best extraction technology was identiifed by the result of L9(33) orthogonal experiment design. The results showed that the inlfuence of various factors were pH>macerating time > Liquid to solid ratio >temperature, the best combination of orthogonal test was A2B1C1. The leaf protein contents extracted from San Deli (Lan Zhou) contrast and MA- 7 were relatively higher at 21.91% and 19.42%respectively.