CAJ | 학술논문

将9只体况良好，体重相近(41.3±1.2kg)，安装有永久性瘤胃瘘管的成都麻羊半同胞羯羊，随机分成3组，饲以稻草基础日粮，分别补饲25%（MSL25）、50%（MSL50）与75%（MSL75）的苜蓿（MSL），在进行增重性能的组合效应研究的同时，采集各组瘤胃液以获取瘤胃原虫和细菌，用4,6-二脒基-2-苯基吲哚（DAPI）标记细菌的荧光强度，进行瘤胃原虫对细菌吞噬作用的组合效应研究。结果显示：（1）瘤胃原虫的细菌吞噬量与吞噬速率均随苜蓿补饲水平的增加而减少，当补饲水平达到50%时，趋于平稳。各补饲水平组的吞噬量在60 min达到饱和，此时吞噬量自高到低的排序为MSL25(381.91)＞MSL50(377.02)＞MSL50(376.47)。（2）MSL25、MSL50与MSL75的日增重分别为19.14 g/d、40.40 g/d与49.26g/d（P＜0.05）；②MSL25、MSL50与MSL75单位增重所需的饲料分别为44.14、23.69与21.26，其中MSL50与MSL75的组间差异不显著（P＞0.05）；MSL25、MSL50与MSL75每千克增重饲料成本分别为58.49元、48.56元与59.01元，其中MSL25与MSL75的组间差异不显著（P＞0.05）。研究结果表明，山羊稻草基础日粮补饲50%的苜蓿可提高瘤胃内蛋白微循环效率、改善增重性能、降低饲料成本。
장9지체황량호，체중상근(41.3±1.2kg)，안장유영구성류위루관적성도마양반동포갈양，수궤분성3조，사이도초기출일량，분별보사25%（MSL25）、50%（MSL50）여75%（MSL75）적목숙（MSL），재진행증중성능적조합효응연구적동시，채집각조류위액이획취류위원충화세균，용4,6-이미기-2-분기신타（DAPI）표기세균적형광강도，진행류위원충대세균탄서작용적조합효응연구。결과현시：（1）류위원충적세균탄서량여탄서속솔균수목숙보사수평적증가이감소，당보사수평체도50%시，추우평은。각보사수평조적탄서량재60 min체도포화，차시탄서량자고도저적배서위MSL25(381.91)＞MSL50(377.02)＞MSL50(376.47)。（2）MSL25、MSL50여MSL75적일증중분별위19.14 g/d、40.40 g/d여49.26g/d（P＜0.05）；②MSL25、MSL50여MSL75단위증중소수적사료분별위44.14、23.69여21.26，기중MSL50여MSL75적조간차이불현저（P＞0.05）；MSL25、MSL50여MSL75매천극증중사료성본분별위58.49원、48.56원여59.01원，기중MSL25여MSL75적조간차이불현저（P＞0.05）。연구결과표명，산양도초기출일량보사50%적목숙가제고류위내단백미순배효솔、개선증중성능、강저사료성본。
Nine healthy Chengdu Ma wether Goats from sire half-sib family with similar weight, were divided into three groups randomly and fed with the rice straw (RS) supplemented with 25% ( MSL25), 50% ( MSL50) and 75% (MSL75) alfalfa hay (Medicago sativa Linn, MSL) respectively to obtain rumen protozoa and bacteria for determinating the phagocytosis of rumen protozoa on bacteria during the study of associative effects on weight gains. The lfuorescence intensity of bacteria labeled by DAPA was tested in this study. The results showed that:A. for the phagocytosis of rumen protozoa on bacteria, the grazing quantity and grazing rate decreased with increasing level of MSL, and then lfattens-off as the supplemented level of MSL reached 50%. The grazing quantity reached saturation at 60 min for 3 mixed forages and ranked from greatest to least as follows: MSL25 (381.91)>MSL50 (377.02)>MSL50 (376.47), the ifgures in parentheses were grazing quantity, and the units were cells/cell. B. for associative effects on weight gains:a. the daily gains of MSL25, MSL50 and MSL50 were 19.14 g/d, 40.40 g/d and 49.26g/d respectively (P<0.05); b. the Feed / Gain of MSL25, MSL50 and MSL50 were 44.14, 23.69 and 21.26 respectively, no signiifcant difference between MSL50 and MSL75 was observed;c. the Feed cost/Gain of MSL25, MSL50 and MSL50 were 58.49 yuan/kg,48.56 yuan/kg and 59.01 yuan/kg respectively, no signiifcant difference between MSL25 and MSL75 was observed. It was concluded that the efifciency of rumen protein microcirculation and the daily gains of goats could be improved when the goat rice straw basic diet supplemented with 50%MSL.