CAJ | 학술논문

以苎麻[Boehmeria nivea （L.) Gaudich.]的纤维成熟期韧皮组织、种子和根组织为试验材料，采用CTAB 法、SDS 法和尿素法提取苎麻基因组 DNA。3种 DNA 提取方法比较，CTAB 法提取的 DNA 质量优于其他两种方法；3种试验材料比较，韧皮组织提取的 DNA 质量最好，其次是种子和根组织。种子提取的DNA 浓度最高。设计梯度试验分析不同研磨时间对韧皮组织和种子总 DNA 提取质量的影响，结果显示韧皮组织研磨4 min、种子研磨6 min 提取的总 DNA 质量较好。
이저마[Boehmeria nivea （L.) Gaudich.]적섬유성숙기인피조직、충자화근조직위시험재료，채용CTAB 법、SDS 법화뇨소법제취저마기인조 DNA。3충 DNA 제취방법비교，CTAB 법제취적 DNA 질량우우기타량충방법；3충시험재료비교，인피조직제취적 DNA 질량최호，기차시충자화근조직。충자제취적DNA 농도최고。설계제도시험분석불동연마시간대인피조직화충자총 DNA 제취질량적영향，결과현시인피조직연마4 min、충자연마6 min 제취적총 DNA 질량교호。
Extraction of high quality genomic DNA from Ramie tissue is the foundation for the study of molecular biology of ramie. In order to study ramie genome by some molecular test, such as PCR , In this study, CTAB, SDS and urea methods were used to extract the ramie genomic DNA from the phloem tissue of ramie fiber mature phase, seed and root tissue respectively. The DNA extraction effect of three different tissues and methods were compared by ultraviolet spectrophotometry and agarose electroforesis. The results showed that the extraction effect of CTAB method is best than the other two method;the extraction effect of the phloem tissue of ramie fiber mature phase is best in three different tissues, follow by the seed and root tissue. The seed is able to extract high quantity genomic DNA in three extraction methods. In addition we study different milling time of the phloem tissue and seeds to total DNA quality effect by used CTAB and SDS methods. The results showed that the phloem tissue grinding for 4min, seed tissue grinding for 6 minutes can get high quality DNA.