湖北农业科学
湖北農業科學
호북농업과학
2014年
11期
2675-2678,2679
,共5页
人类%组成型外显子%可变外显子%密码子偏性%聚类
人類%組成型外顯子%可變外顯子%密碼子偏性%聚類
인류%조성형외현자%가변외현자%밀마자편성%취류
human%constitutive exons%alternative exons%codon bias%clustering
通过计算人类组成型外显子和可变外显子的 RSCU 值，对两种外显子的 RSCU 值进行了比较。结果表明，除了 UAG、UGA、UAA 3个终止密码子外，两种外显子的密码子偏性都是相同的。对30条可变外显子和30条组成型外显子序列的 RSCU 值进行聚类分析，聚类结果不能把两种外显子区别开，证明两种外显子之间的密码子偏性是相同的。对这60条序列的 HI 进行了聚类分析，聚类结果没有把两种外显子分开。所以外显子序列中没有组成型剪接和可变剪接的信息。
通過計算人類組成型外顯子和可變外顯子的 RSCU 值，對兩種外顯子的 RSCU 值進行瞭比較。結果錶明，除瞭 UAG、UGA、UAA 3箇終止密碼子外，兩種外顯子的密碼子偏性都是相同的。對30條可變外顯子和30條組成型外顯子序列的 RSCU 值進行聚類分析，聚類結果不能把兩種外顯子區彆開，證明兩種外顯子之間的密碼子偏性是相同的。對這60條序列的 HI 進行瞭聚類分析，聚類結果沒有把兩種外顯子分開。所以外顯子序列中沒有組成型剪接和可變剪接的信息。
통과계산인류조성형외현자화가변외현자적 RSCU 치，대량충외현자적 RSCU 치진행료비교。결과표명，제료 UAG、UGA、UAA 3개종지밀마자외，량충외현자적밀마자편성도시상동적。대30조가변외현자화30조조성형외현자서렬적 RSCU 치진행취류분석，취류결과불능파량충외현자구별개，증명량충외현자지간적밀마자편성시상동적。대저60조서렬적 HI 진행료취류분석，취류결과몰유파량충외현자분개。소이외현자서렬중몰유조성형전접화가변전접적신식。
RSCU of the constitutive exons and alternative exons of human were calculated. Two kinds of exons RSCU values were compared. The results showed that every codon bias was the same in two kinds of exons, except three stop codons (UAG,UGA,UAA). 30 constitutive exon sequences and 30 alternative exon sequences were selected to calculate RSCU values of them. The cluster analysis did not distinguish two kinds of exons, proving that the codon biases were the same between the two kinds of exons. HI of the 60 sequences were calculated and used to cluster analysis. It was not able to separate the two kinds of exons. It is indicated that there is no constitutive splicing and alternative splicing information in the exon se-quences.
