CAJ | 학술논문

采用超声波和微波技术提取大火草[Anemone tomentosa（Maxim.)Péi]根、茎、叶中的多糖，确定超声波醇提为最佳提取方法，用苯酚-硫酸法测定其多糖含量。通过单因素试验和响应面分析法，研究了料液比、温度、提取时间对多糖提取率的影响。结果表明，提取时间和温度是影响大火草多糖提取率的主要因素，试验稳定性、精密度良好，提取大火草多糖的最佳条件为温度80℃，提取时间50 min，料液比1：100（m：V)。利用最佳提取工艺得到根、茎、叶中的多糖含量分别为5.16%、1.61%、7.16%。
채용초성파화미파기술제취대화초[Anemone tomentosa（Maxim.)Péi]근、경、협중적다당，학정초성파순제위최가제취방법，용분분-류산법측정기다당함량。통과단인소시험화향응면분석법，연구료료액비、온도、제취시간대다당제취솔적영향。결과표명，제취시간화온도시영향대화초다당제취솔적주요인소，시험은정성、정밀도량호，제취대화초다당적최가조건위온도80℃，제취시간50 min，료액비1：100（m：V)。이용최가제취공예득도근、경、협중적다당함량분별위5.16%、1.61%、7.16%。
The polysaccharides were extracted from the roots, stems and leaves of Anemone tomentosa (Maxim.) Péi with ul-trasonic and microwave extraction. The ultrasound-assisted extraction with ethanol was the best method. Polysaccharides were measured by phenol-sulfuric acid method. The effects of material-liquid ratio, extraction temperature and time on the extrac-tion of polysaccharides were studied with single-factor experiment and response surface methodology. The results showed that the temperature and the time were the main factors. The optimal condition of polysaccharides extraction was temperature of 80℃,extraction time of 50 min, material-liquid ratio of 1:100 (m:V). Under the optimal conditions, the contents of polysaccha-rides in roots, stems and the leaves were 5.16%,1.61% and 7.16%,respectively.