CAJ | 학술논문

建立了同时测定人参蜂王浆口服液中15种抗生素药物（6种喹诺酮类、4种四环素类、3种大环内酯类、2种林可胺类）残留的高效液相色谱-串联质谱（LC-MS/MS）检测方法。该方法采用pH 8.0磷酸盐缓冲液提取样品，提取液经HLB固相萃取柱净化。以甲醇-乙腈-0.1%甲酸作为流动相，C18色谱柱对15种药物进行分离，电喷雾正离子多反应监测模式进行检测。方法检测限为0.02 ng/mL～0.9 ng/mL，线性范围0.08 ng/mL～20 ng/mL，加标回收率为71.8%～87.8%，相对标准偏差为2.2%～7.9%（n=9）。该方法准确、灵敏、简便，适用于人参蜂王浆口服液中四环素等药物残留的测定。
건립료동시측정인삼봉왕장구복액중15충항생소약물（6충규낙동류、4충사배소류、3충대배내지류、2충림가알류）잔류적고효액상색보-천련질보（LC-MS/MS）검측방법。해방법채용pH 8.0린산염완충액제취양품，제취액경HLB고상췌취주정화。이갑순-을정-0.1%갑산작위류동상，C18색보주대15충약물진행분리，전분무정리자다반응감측모식진행검측。방법검측한위0.02 ng/mL～0.9 ng/mL，선성범위0.08 ng/mL～20 ng/mL，가표회수솔위71.8%～87.8%，상대표준편차위2.2%～7.9%（n=9）。해방법준학、령민、간편，괄용우인삼봉왕장구복액중사배소등약물잔류적측정。
A HPLC-MS/MS method was established for simultaneous determination of 15 drug residues (6 quinolones,4 tetracyclines,3 macrolides,lincomycin and clindamycin)in Ginseng and Royal Jelly Oral Liquid. Samples were extracted with phosphate buffer solution (pH=8.0)and then purified by Oasis HLB solid phase extraction column. Chromatographic separation was carried out on a C18 column,the mobile phase is methanol-acetonitrile-0.1%formic acid water solution with gradient elution, then detected by MS/MS in the ESI(+)-MRM mode.The limits of detection for the method were 0.02 ng/mL-0.9 ng/mL,The linear plots were obtained between 0.08 ng/mL-20 ng/mL,recoveries ranged from 71.8%-87.8%,and relative standard deviations were in the range of 2.2%-7.9%(n=9).