CAJ | 학술논문

目的：探讨薄层液基脱落细胞检查（TCT）、高危型乳头状瘤病毒（HPV）DNA检测及脱落细胞p16蛋白免疫细胞化学检测在宫颈癌早期筛查中的应用价值。方法对TCT、HC2- HPV- DNA检测至少一项异常的815例患者，利用剩余细胞学标本进行p16免疫组化检测，同时行阴道镜及病理组织学检查进行对照。结果 HC2- HPV- DNA的阳性率和宫颈脱落细胞中p16表达阳性率随着宫颈病变严重程度的增加而明显升高（P＜0.01）。HC2- HPV- DNA+TCT检测提高了宫颈癌及癌前病变的检出率，p16免疫细胞化学检查则提高了检测的特异度。结论在TCT和HC2- HPV- DNA检测的基础上，联合应用脱落细胞p16免疫细胞化学染色，可提高宫颈癌早期筛查的准确性。
목적：탐토박층액기탈락세포검사（TCT）、고위형유두상류병독（HPV）DNA검측급탈락세포p16단백면역세포화학검측재궁경암조기사사중적응용개치。방법대TCT、HC2- HPV- DNA검측지소일항이상적815례환자，이용잉여세포학표본진행p16면역조화검측，동시행음도경급병리조직학검사진행대조。결과 HC2- HPV- DNA적양성솔화궁경탈락세포중p16표체양성솔수착궁경병변엄중정도적증가이명현승고（P＜0.01）。HC2- HPV- DNA+TCT검측제고료궁경암급암전병변적검출솔，p16면역세포화학검사칙제고료검측적특이도。결론재TCT화HC2- HPV- DNA검측적기출상，연합응용탈락세포p16면역세포화학염색，가제고궁경암조기사사적준학성。
Objective To evaluate the application of thin cytology test(TCT) and Hybrid Capture 2- high risk HPV- DNA test (HC2- HPV- DNA) combined with p16 immunocytochemical staining in screening of early cervical cancer. Methods Total 815 women underwent TCT and HC2- HPV- DNA testing, the specimens with at least one abnormal finding were subject to p16 im-munocytochemical examination, and biopsy under colposcopy was also performed for these patients. Results The positive rate of both HC2- HPV- DNA test and p16 expression increased with the degree of cervical lesions(P<0.01). Simultaneous detection of TCT and HC2- HPV- DNA increased the detection rate of cervical cancers and cancer precursor lesions. Incorporation of these two tests with p16 Immunocytochemical staining improved the specificity of detection. Conclusion TCT and HC2- HPV- DNA detec-tion combined with immunocytochemical staining for p16 can improve the specificity of cervical cancer screening.