CAJ | 학술논문

比较石墨炉原子吸收法测定全血标本中铅的3种前处理方法优劣，从中筛选一种更简便、准确、灵敏的方法，从而达到提高检测质量，降低工作强度的目的。方法采用酸脱蛋白法、混合基体改进剂法、微波消解法对无铅接触史的健康成年人全血标本进行前处理，分别用石墨炉原子吸收法检测，对检测结果进行统计分析，比较标准曲线线性、相关系数（r）、相对标准偏差（RSD％）、准确度等指标。结果3种方法标准曲线 r分别为0．9965、0．9942、0．9995；检出限分别为5．4、3．6、2．7μg/L ；RSD％分别为4．1、4．5、2．8；回收率分别为95．7％～103．3％、98．7％～104．5％、98．3％～102．3％。结论微波消解处理全血标本各项指标均令人满意，是测量血铅较好的前处理方法。
비교석묵로원자흡수법측정전혈표본중연적3충전처리방법우렬，종중사선일충경간편、준학、령민적방법，종이체도제고검측질량，강저공작강도적목적。방법채용산탈단백법、혼합기체개진제법、미파소해법대무연접촉사적건강성년인전혈표본진행전처리，분별용석묵로원자흡수법검측，대검측결과진행통계분석，비교표준곡선선성、상관계수（r）、상대표준편차（RSD％）、준학도등지표。결과3충방법표준곡선 r분별위0．9965、0．9942、0．9995；검출한분별위5．4、3．6、2．7μg/L ；RSD％분별위4．1、4．5、2．8；회수솔분별위95．7％～103．3％、98．7％～104．5％、98．3％～102．3％。결론미파소해처리전혈표본각항지표균령인만의，시측량혈연교호적전처리방법。
Objective To compare three specimen pretreatment methods for measuring lead content in whole blood by graphite furnace atomic absorption spectrometry .Methods Whole blood of healthy adults without lead ex‐posure history were pretreated by using acid deproteinized method ,mixed matrix modifier method ,and microwave di‐gestion method ,and then detected for lead content by using graphite furnace atomic absorption spectrometry .All re‐sults were statistically analyzed to compare indicators ,including standard curve′s linear ,correlation coefficient ,rela‐tive standard deviation (RSD% ) ,accuracy and so on .Results Correlation coefficients of standard curve of the three methods were 0 .996 5 ,0 .994 2 and 0 .999 5 ,the minimum detectable concentration were 5 .4 ,3 .6 and 2 .7 μg/L , RSD% were 4 .1 ,4 .5 and 2 .8 ,and the recovery rates were 95 .7% -103 .3% ,98 .7% -104 .5% and 98 .3% -102 .3% .Conclusion The evaluation indicators of microwave digestion method could be satisfactory ,which might be an ideal method for pretreatment of blood specimens for detection of lead content .