CAJ | 학술논문

为给“瓯雪60天”制种中核不育系母本9901A组培快繁提供依据，以9901A花球表面花枝为外植体，采用正交设计方法对培养基中TDZ、BA、NAA、2，4-D 4种激素浓度配比进行筛选。结果表明：MS+6-BA0.5mg/L+NAA0.001mg/L芽诱导率最大，为94%，但每外植体平均诱导芽数为1.1个；MS+TDZ0.1mg/L+NAA0.001mg/L每外植体平均诱导芽数最大，为10个，但芽诱导率为50%。TDZ和NAA浓度适中促进外植体平均芽数的诱导，过高则抑制外植体平均芽数的诱导；2，4-D 和6-BA总体表现为随浓度增加而抑制外植体平均芽数的诱导。
위급“구설60천”제충중핵불육계모본9901A조배쾌번제공의거，이9901A화구표면화지위외식체，채용정교설계방법대배양기중TDZ、BA、NAA、2，4-D 4충격소농도배비진행사선。결과표명：MS+6-BA0.5mg/L+NAA0.001mg/L아유도솔최대，위94%，단매외식체평균유도아수위1.1개；MS+TDZ0.1mg/L+NAA0.001mg/L매외식체평균유도아수최대，위10개，단아유도솔위50%。TDZ화NAA농도괄중촉진외식체평균아수적유도，과고칙억제외식체평균아수적유도；2，4-D 화6-BA총체표현위수농도증가이억제외식체평균아수적유도。
In order to provide a certain reference for rapid propagation of the female parent 9901A(genic male sterile lines) of "Ou xue 60 days",concentrations of TDZ,BA,NAA,2,4-D on multiple shoots induc-tion were screening by orthogonal design method.The results show that,the budding induction rate of MS+6-BA0.5mg/L+NAA0.001mg/L was the highest (94%) with budding induction number 1.1 per explants;and the bud-ding induction number of MS+TDZ0.1mg/L+NAA0.001mg/L was the highest(10 per plant)with budding induction rate 50%.It was suggested that TDZ and NAA had a promoting effect on budding induction with moderate concentration.The effect of 2,4-D and 6-BA on budding induction was reduced as the concentration in-creased.