CAJ | 학술논문

目的：改进大鼠经皮穿刺腰骶部鞘内置管技术，探索更加简便、快捷、安全、有效的大鼠鞘内给药的新方法。方法：健康雄性大鼠60只，按随机数字表法分为经寰枕膜鞘内置管组（C组，n=30）和经皮穿刺腰骶部鞘内置管组（T组，n=30）。戊巴比妥钠腹腔麻醉后，C组大鼠接受经皮切开寰枕膜鞘内置管操作，T组大鼠接受在L6~S1间隙，用20 G穿刺针行经皮穿刺腰骶部鞘内置管操作。两组大鼠在操作成功后，均经导管注入2%利多卡因10μL。观察并比较两组大鼠置管成功只数、瘫痪只数以及死亡只数。结果：与C组比较，T组大鼠鞘内置管成功率明显提高，术后瘫痪、死亡率明显减少，差异均有统计学意义（P<0.05）。结论：经皮穿刺腰骶部鞘内置管技术是一项简便、快捷、安全、有效的鞘内给药方法。
목적：개진대서경피천자요저부초내치관기술，탐색경가간편、쾌첩、안전、유효적대서초내급약적신방법。방법：건강웅성대서60지，안수궤수자표법분위경환침막초내치관조（C조，n=30）화경피천자요저부초내치관조（T조，n=30）。무파비타납복강마취후，C조대서접수경피절개환침막초내치관조작，T조대서접수재L6~S1간극，용20 G천자침행경피천자요저부초내치관조작。량조대서재조작성공후，균경도관주입2%리다잡인10μL。관찰병비교량조대서치관성공지수、탄탄지수이급사망지수。결과：여C조비교，T조대서초내치관성공솔명현제고，술후탄탄、사망솔명현감소，차이균유통계학의의（P<0.05）。결론：경피천자요저부초내치관기술시일항간편、쾌첩、안전、유효적초내급약방법。
To explore a simple,safe,fast and effective method of intrathecal application by studying the method of intrathecal catheterization via lumbosacral puncture in rats.Method:60 healthy rats were randomly divided into the intrathecal catheterization through the atlantooccipital membrane group(the group C,n=30)and the intrathecal catheterization through lumbosacral puncture group(the group T,n=30). All the rats in the two groups received abdominal anesthesia by pentobarbital natrium. The group C received intrathecal catheterization by the atlantooccipital membrane. The group T received intrathecal catheterization by 20 G needle in L6-S1 interspace. Then,all the rats in the two groups were administrated 2% lidocaine hydrochloride 10 μL via the catheter. The number of successful catheterization,paralysis and death were compared in the two groups.Result:Compared with the group C,the success rate of catheterization was significantly increased,the incidence of death and paralysis were significantly decreased in the group T,the differences were statistically significant(P<0.05).Conclusion:Intrathecal catheterization by lumbosacral puncture is an effective and safe intrathecal catheterization method for rats undergoing scientific experiments.