中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
18期
30-32
,共3页
脊神经选择性结扎%镜像痛%模型
脊神經選擇性結扎%鏡像痛%模型
척신경선택성결찰%경상통%모형
Spinal nerve selective ligation%Mirror-image pain%Model
目的：研究行为学观察和热辐射缩足反应潜伏期（PWTL）及机械缩足反应潜伏期（MWT）实验筛选建立脊神经选择性结扎（SNL）镜像痛大鼠模型。方法：选取100只成年雄性SD大鼠，将其随机分为SNL组90只和假手术组（Sham组）10只，于术前1 d和术后1、3、7、14、21、28 d进行行为学观察和双足PWTL及MWT实验检测。结果：两组术前行为学观察、PWTL及MWT值比较差异均无统计学意义（P>0.05），术后SNL组手术足均出现添足、咬足等行为学现象，且PWTL值和MWT值术后1~28 d均明显低于Sham组，差异均有统计学意义（P<0.05）。SNL组有14只大鼠为手术足，于术后7 d出现添足、咬足等行为学现象，术后14、21、28 d的MWT值均明显低于Sham组，且术后21、28 d的PWTL值均明显低于Sham组，差异均有统计学意义（P<0.05）。结论：大鼠SNL镜像痛模型筛选和建立成功。
目的：研究行為學觀察和熱輻射縮足反應潛伏期（PWTL）及機械縮足反應潛伏期（MWT）實驗篩選建立脊神經選擇性結扎（SNL）鏡像痛大鼠模型。方法：選取100隻成年雄性SD大鼠，將其隨機分為SNL組90隻和假手術組（Sham組）10隻，于術前1 d和術後1、3、7、14、21、28 d進行行為學觀察和雙足PWTL及MWT實驗檢測。結果：兩組術前行為學觀察、PWTL及MWT值比較差異均無統計學意義（P>0.05），術後SNL組手術足均齣現添足、咬足等行為學現象，且PWTL值和MWT值術後1~28 d均明顯低于Sham組，差異均有統計學意義（P<0.05）。SNL組有14隻大鼠為手術足，于術後7 d齣現添足、咬足等行為學現象，術後14、21、28 d的MWT值均明顯低于Sham組，且術後21、28 d的PWTL值均明顯低于Sham組，差異均有統計學意義（P<0.05）。結論：大鼠SNL鏡像痛模型篩選和建立成功。
목적：연구행위학관찰화열복사축족반응잠복기（PWTL）급궤계축족반응잠복기（MWT）실험사선건립척신경선택성결찰（SNL）경상통대서모형。방법：선취100지성년웅성SD대서，장기수궤분위SNL조90지화가수술조（Sham조）10지，우술전1 d화술후1、3、7、14、21、28 d진행행위학관찰화쌍족PWTL급MWT실험검측。결과：량조술전행위학관찰、PWTL급MWT치비교차이균무통계학의의（P>0.05），술후SNL조수술족균출현첨족、교족등행위학현상，차PWTL치화MWT치술후1~28 d균명현저우Sham조，차이균유통계학의의（P<0.05）。SNL조유14지대서위수술족，우술후7 d출현첨족、교족등행위학현상，술후14、21、28 d적MWT치균명현저우Sham조，차술후21、28 d적PWTL치균명현저우Sham조，차이균유통계학의의（P<0.05）。결론：대서SNL경상통모형사선화건립성공。
To study choice and establishment of mirror-image pain model of selective spinal nerve ligation(SNL)in rats through the behavior observation,paw withdrawal thermal latency(PWTL)and mechanical withdrawal threshold(MWT)test.Method:100 adult male SD rats were randomly divided into the SNL group for 90 rats and the sham operation group(Sham group)for 10 rats.All rats were observed behaviors,detected PWTL and MWT at the 1 day before operation and the 1,3,7,14,21,28 day after operation.Result:There had no significant differences in behavioral observation,PWTL and MWT values between the two groups at the 1 day before operation(P>0.05). Post-operation,SNL group’s operated-foot appeared hit feet,bite feet behavioral phenomenon,and the PWTL values and MWT values were significantly lower compared with Sham group at 1-28 d post-operation,the differences were statistically significant(P<0.05).There were 14 rats of the SNL group appeared hit feet,bite feet behavioral phenomenon in 7 d post-operation at un-operation foot,appeared MWT values were significantly lower compared with Sham group at 14,21,28 d post-operation and PWTL values were significantly lower compared with Sham group at 21,28 d post-operation,the differences were statistically significant(P<0.05).Conclusion:The choice and establishment of mirror-image pain model of SNL rats are successful.
