医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2014年
18期
131-132
,共2页
新鲜抗凝全血%血液分析仪%比对
新鮮抗凝全血%血液分析儀%比對
신선항응전혈%혈액분석의%비대
Fresh blood%Hematology analyzer
目的应用正常人新鲜抗凝全血对我院1月份实验室多台血液分析仪进行比对实验，评价各血液分析仪各参数之间是否具有可比性，本次实验研究的是在血液分析过程中临床意义比较大的几个参数：白细胞(WBC)红细胞(RBC)、血红蛋白(HGB)、红细胞压积(HCT)、血小板(PLT)。方法选择本实验室日本Sysmex公司生产的XT一2000i(有配套可溯源的校准物和质控品)血细胞分析仪为参比仪器。先对一正常新鲜抗凝全血定值。用于校准其它血细胞分析仪。校准合格后每日选取一门诊体检人正常新鲜全血在各台仪器上进行测定，以参比仪器所测结果为靶值，其它两台仪器所测结果与参比仪器所测结果相比较，计算出WBC﹑RBC﹑HGB﹑HCT﹑PLT的差异百分比。当差异百分比在允许范围内时表示在控，当差异百分比超出允许范围时表示失控。结果我实验室XT一2000i检测结果与ABX-PENTRA120，迈瑞BC-5500比较，WBC、RBC、HGB、HCT、PLT相关性较好，都在CLIA'88允许误差范围的l/4控制限范围内，实验结果可靠具有可比性。结论同一实验室不同仪器间利用新鲜抗凝全血标本进行比对测定，利用Excel数据点折线图的功能，对差异百分数作图动态观察。及时发现偶然误差和系统误差。查找原因并作相应处理，可以使多台血细胞分析仪的结果间具有可比性，减小差异率，达到了科学又经济的目的。
目的應用正常人新鮮抗凝全血對我院1月份實驗室多檯血液分析儀進行比對實驗，評價各血液分析儀各參數之間是否具有可比性，本次實驗研究的是在血液分析過程中臨床意義比較大的幾箇參數：白細胞(WBC)紅細胞(RBC)、血紅蛋白(HGB)、紅細胞壓積(HCT)、血小闆(PLT)。方法選擇本實驗室日本Sysmex公司生產的XT一2000i(有配套可溯源的校準物和質控品)血細胞分析儀為參比儀器。先對一正常新鮮抗凝全血定值。用于校準其它血細胞分析儀。校準閤格後每日選取一門診體檢人正常新鮮全血在各檯儀器上進行測定，以參比儀器所測結果為靶值，其它兩檯儀器所測結果與參比儀器所測結果相比較，計算齣WBC﹑RBC﹑HGB﹑HCT﹑PLT的差異百分比。噹差異百分比在允許範圍內時錶示在控，噹差異百分比超齣允許範圍時錶示失控。結果我實驗室XT一2000i檢測結果與ABX-PENTRA120，邁瑞BC-5500比較，WBC、RBC、HGB、HCT、PLT相關性較好，都在CLIA'88允許誤差範圍的l/4控製限範圍內，實驗結果可靠具有可比性。結論同一實驗室不同儀器間利用新鮮抗凝全血標本進行比對測定，利用Excel數據點摺線圖的功能，對差異百分數作圖動態觀察。及時髮現偶然誤差和繫統誤差。查找原因併作相應處理，可以使多檯血細胞分析儀的結果間具有可比性，減小差異率，達到瞭科學又經濟的目的。
목적응용정상인신선항응전혈대아원1월빈실험실다태혈액분석의진행비대실험，평개각혈액분석의각삼수지간시부구유가비성，본차실험연구적시재혈액분석과정중림상의의비교대적궤개삼수：백세포(WBC)홍세포(RBC)、혈홍단백(HGB)、홍세포압적(HCT)、혈소판(PLT)。방법선택본실험실일본Sysmex공사생산적XT일2000i(유배투가소원적교준물화질공품)혈세포분석의위삼비의기。선대일정상신선항응전혈정치。용우교준기타혈세포분석의。교준합격후매일선취일문진체검인정상신선전혈재각태의기상진행측정，이삼비의기소측결과위파치，기타량태의기소측결과여삼비의기소측결과상비교，계산출WBC﹑RBC﹑HGB﹑HCT﹑PLT적차이백분비。당차이백분비재윤허범위내시표시재공，당차이백분비초출윤허범위시표시실공。결과아실험실XT일2000i검측결과여ABX-PENTRA120，매서BC-5500비교，WBC、RBC、HGB、HCT、PLT상관성교호，도재CLIA'88윤허오차범위적l/4공제한범위내，실험결과가고구유가비성。결론동일실험실불동의기간이용신선항응전혈표본진행비대측정，이용Excel수거점절선도적공능，대차이백분수작도동태관찰。급시발현우연오차화계통오차。사조원인병작상응처리，가이사다태혈세포분석의적결과간구유가비성，감소차이솔，체도료과학우경제적목적。
Objective Applying normal person fresh anticoagulant blood carries out comparison for several hematology analyzers which come from our laboratory in January,and evaluates whether there have been comparability among the parameter of the hematology analyzers.This experiment researches several parameters standing for the great clinical significance in the process of hemanalysis.Methods The Blood Cel Analyzer -XT-2000i (there are the calibrated mat er and the control materials that are assorted and traced to the source) made in SYSMEX corporation of Japan is chosen to be reference instrument.First,fixing numerical value for fresh anticoagulant blood is so as to calibrate another Blood Cell Analyzers.After calibration is qualified,the normal and fresh blood from an outpatient wil be chosen every day in order to be made determination on the instruments.Meanwhile,we regard the result measured by reference instrument as target value,compare it with the measured result of another two instruments and calculate discrepant percentage of WBC,RBC,HGB,HCT,PCT.When discrepant percentage comes within allowed range the result could be under control;when discrepant percentage exceeds al owed range,it could be out of control.Results Comparing the measured result of XT-2000i in the laboratory with one of ABX-PENTRA120,MINDARY BC-5500,WBC,RBC,HGB,HCT,PCT all possess the bet er pertinence,which is in 1/4 control range of permit ed error range of CLIA'88.The laboratory result is so reliable and has comparability.Conclusion Among dif erent instruments in the same laboratory,making use of fresh anticoagulant blood specimen to advance comparison determination and taking advantage of the function of line chart made up of Excel's data points to dynamical y examine discrepant percentage,quickly find accidentalerrorsystematic residuals,ask for reasons in order to carry out the homologous solution could create comparability among the results of several Blood Cel Analyzers,minish discrepant ratio so as to achieve scientific objective and economic purpose.
