CAJ | 학술논문

目的了解2009-2011年惠州市手足口病流行的病原及流行情况.方法采集253份2009-2011年手足口病患儿的标本,采用荧光定量PCR法对患者标本进行核酸检测,对部分结果阳性的样品进行基因序列分型.结果 253份样品中,总肠道病毒核酸(PE)检测阳性156份,其中CoxA16核酸阳性41份,EV71核酸阳性92份；2009年手足口病流行的病原以CoxA16型为主(69.0％);2010年和2011年均以EV71型为主(分别为78.1％和67.5％)；30份EV71型病毒的基因测序分型均为EV71-C4a型,2例EV阳性病例标本的病原基因型为ECHO3型；肠道病毒阳性率6岁以下儿童占阳性病例的93.5％ (146/156),3岁以下幼童占阳性病例的73.0％ (114/156).结论本地区2009年手足口病流行的病原以CoxA16型为主,2010年和2011年以EV71型为主,3年流行的EV71病原基因型主要为EV71-C4a型,在其它肠道病毒阳性的标本中检出基因型为ECHO3的病原；3岁以下的幼童为EV71感染的高危人群,在夏秋季节做好手足口病的防控工作很有必要.
목적 료해2009-2011년혜주시수족구병류행적병원급류행정황.방법 채집253빈2009-2011년수족구병환인적표본,채용형광정량PCR법대환자표본진행핵산검측,대부분결과양성적양품진행기인서렬분형.결과 253빈양품중,총장도병독핵산(PE)검측양성156빈,기중CoxA16핵산양성41빈,EV71핵산양성92빈；2009년수족구병류행적병원이CoxA16형위주(69.0％);2010년화2011년균이EV71형위주(분별위78.1％화67.5％)；30빈EV71형병독적기인측서분형균위EV71-C4a형,2례EV양성병례표본적병원기인형위ECHO3형；장도병독양성솔6세이하인동점양성병례적93.5％ (146/156),3세이하유동점양성병례적73.0％ (114/156).결론 본지구2009년수족구병류행적병원이CoxA16형위주,2010년화2011년이EV71형위주,3년류행적EV71병원기인형주요위EV71-C4a형,재기타장도병독양성적표본중검출기인형위ECHO3적병원；3세이하적유동위EV71감염적고위인군,재하추계절주호수족구병적방공공작흔유필요.
Objective To investigate the pathogens and prevalence of hand-foot-mouth disease (HFMD) in Huizhou during 2009 to 2011.Methods 253 specimens were collected from the children with HFMD and then were detected using fluorescence quantitative PCR.Some samples with positive results were typed by gene sequencing.Results 156 of 253 samples were PE positive.41 of the 156 samples were CoxA16 and 92 were EV71.CoxA16 (69％) was the major pathogen in 2009,while EV71 was prevalent in 2010 and 2011 (78.1％ and 67.5％,respectively).The types of EV71 were EV71-C4a for 30 and ECHO3 for 2.The positive rate of intestinal virus accounted for 93.5％ (146/156) in children aged less than 6 and was 73.0％(114/156) in those aged less than 3.Conclusions CoxA16 is the major pathogen for hand-food-mouth disease in 2009 and EV71 is the predominant pathogen in 2010 and 2011.EV71-C4a is the main type of EV71 in the past three years in Huizhou.Children younger than 3 are at high risk for EV71 infection.Prevention and control of hand-foot-mouth disease in summer and fall is a necessity.