CAJ | 학술논문

目的：研究聚乳酸/乙醇酸可吸收医用膜对兔腹腔术后粘连的预防作用。方法日本大耳白兔66只，随机分成3组，正常对照组n=6，模型对照组n=30，PLGA膜组n=30。除正常对照组外均采用多因素法复制腹腔术后粘连模型，PLGA膜组在盲肠创面贴敷PLGA膜。术后1、2、4、6、12周后打开腹腔，评定腹膜粘连程度，并对盲肠组织行羟脯氨酸水平测定和HE组织病理学观察。结果(1)模型对照组的粘连程度和羟脯氨酸水平均明显高于正常对照组(P<0.05,P<0.01)，且PLGA膜组的粘连程度和羟脯氨酸水平均显著低于模型对照组(P<0.05)。(2) HE染色显示模型对照组盲肠浆膜层有明显的炎症细胞浸润和纤维组织增生。PLGA膜能抑制纤维细胞的增生，炎症细胞浸润减少和胶原纤维明显减少。结论 PLGA膜能抑制成纤维细胞的增生和胶原蛋白的形成，减轻了兔术后粘连发生的程度和范围。
목적：연구취유산/을순산가흡수의용막대토복강술후점련적예방작용。방법일본대이백토66지，수궤분성3조，정상대조조n=6，모형대조조n=30，PLGA막조n=30。제정상대조조외균채용다인소법복제복강술후점련모형，PLGA막조재맹장창면첩부PLGA막。술후1、2、4、6、12주후타개복강，평정복막점련정도，병대맹장조직행간포안산수평측정화HE조직병이학관찰。결과(1)모형대조조적점련정도화간포안산수평균명현고우정상대조조(P<0.05,P<0.01)，차PLGA막조적점련정도화간포안산수평균현저저우모형대조조(P<0.05)。(2) HE염색현시모형대조조맹장장막층유명현적염증세포침윤화섬유조직증생。PLGA막능억제섬유세포적증생，염증세포침윤감소화효원섬유명현감소。결론 PLGA막능억제성섬유세포적증생화효원단백적형성，감경료토술후점련발생적정도화범위。
Objective To investigate the effects of PLGA absorbable membrane in prevention of postoperative abdominal adhesion in rabbits.Methods 66 Japanese white rabbits were randomly divided into three groups: the normal control groupn=6, model control groupn=30 and PLGA groupn=30. Rabbits were received multifactor methods to establish postoperative abdominal adhesion models except for normal control group. The cecum wound was covered PLGA membrane in the PLGA group. At postoperative 1, 2, 4, 6 and 12 weeks, the abdominal cavities were reopened and the adhesive severity was graded blindly, and the hydroxyproline level in cecum tissue was measured and the cecum histopathology was observed. Results (1) the degree of adhesion and hydroxyproline level in model control group were significantly higher than those of normal control group (P<0.05,P<0.01), while the degree of adhesion and hydroxyproline level in PLGA group were significantly lower than those of model control group (P<0.05). (2) HE staining showed that cecum serosa had obviously inflammatory cellinfiltration andfibroblast proliferation, while PLGA could inhibit fibroblast proliferation and reduce the inflammatory cellinfiltration and colagen.Conclusion PLGA absorbable membrane can inhibit fibroblast proliferation and collagen to prevent the experimental postoperative peritoneal adhesions.