药学实践杂志
藥學實踐雜誌
약학실천잡지
THE JOURNAL OF PHARMACEUTICAL PRACTICE
2014年
5期
352-353,356
,共3页
余学英%常明泉%陈芳%王刚
餘學英%常明泉%陳芳%王剛
여학영%상명천%진방%왕강
维地红涂剂%多糖%含量测定
維地紅塗劑%多糖%含量測定
유지홍도제%다당%함량측정
Verdihong paint%polysaccharide%content determination
目的：建立维地红涂剂中多糖的含量测定方法。方法用无水葡萄糖为对照品，0．2％蒽酮-硫酸试液为染色剂，紫外分光光度法测定，测定波长620 nm。结果多糖在1．9～34．2μg/ml范围内线性关系良好，Y＝0．033X-0．0067（r＝0．9999），回收率为98．6％，RSD＝1．06％（n＝6）。结论所建方法便捷、重现性好，适用于维地红涂剂中多糖的含量测定。
目的：建立維地紅塗劑中多糖的含量測定方法。方法用無水葡萄糖為對照品，0．2％蒽酮-硫痠試液為染色劑，紫外分光光度法測定，測定波長620 nm。結果多糖在1．9～34．2μg/ml範圍內線性關繫良好，Y＝0．033X-0．0067（r＝0．9999），迴收率為98．6％，RSD＝1．06％（n＝6）。結論所建方法便捷、重現性好，適用于維地紅塗劑中多糖的含量測定。
목적：건립유지홍도제중다당적함량측정방법。방법용무수포도당위대조품，0．2％은동-류산시액위염색제，자외분광광도법측정，측정파장620 nm。결과다당재1．9～34．2μg/ml범위내선성관계량호，Y＝0．033X-0．0067（r＝0．9999），회수솔위98．6％，RSD＝1．06％（n＝6）。결론소건방법편첩、중현성호，괄용우유지홍도제중다당적함량측정。
Objective To establish the determination method of the polysaccharide in Verdihong paint .Methods The anhy-drous dextrose was used as the control , 0.2%anthrone-sulfuric acid as the stain , the content of polysaccharide was determined by ul-traviolet spectrophotometer at the wavelength of 620 nm.Results The polysaccharide curve was linear within a range of 1.9~34.2μg/ml, linear equation was Y=0.033 X-0.006 7 (r=0.999 9), the average recovery was 98.6% with RSD of 1.06%(n=6). Conclusion This method was convenient , stable and coud be used to assay content of the polysaccharide in Verdihong paint .
