物探与化探
物探與化探
물탐여화탐
GEOPHYSICAL AND GEOCHEMICAL EXPLORATION
2014年
5期
976-980
,共5页
任春%汤玉平%何晋发%高俊阳%许科伟
任春%湯玉平%何晉髮%高俊暘%許科偉
임춘%탕옥평%하진발%고준양%허과위
微生物勘探%分子生物学%基因定量%油气勘探
微生物勘探%分子生物學%基因定量%油氣勘探
미생물감탐%분자생물학%기인정량%유기감탐
microbial prospecting%molecular biology%gene quantitative technique%oil and gas prospecting
针对特定功能基因发展起来的油气微生物免培养检测技术,与传统培养法相比具有快速、稳定、特异性高的特点,非常适合工业化测试。在沾化凹陷邵家洼陷陈22井区,对钻孔、剖面以及区域所采集的微生物土壤样品进行检测研究,结果显示,利用基因定量技术检测到的油气微生物异常与油气田有极好的对应关系,证明发展油气微生物分子勘探技术技术具有广阔的远景。
針對特定功能基因髮展起來的油氣微生物免培養檢測技術,與傳統培養法相比具有快速、穩定、特異性高的特點,非常適閤工業化測試。在霑化凹陷邵傢窪陷陳22井區,對鑽孔、剖麵以及區域所採集的微生物土壤樣品進行檢測研究,結果顯示,利用基因定量技術檢測到的油氣微生物異常與油氣田有極好的對應關繫,證明髮展油氣微生物分子勘探技術技術具有廣闊的遠景。
침대특정공능기인발전기래적유기미생물면배양검측기술,여전통배양법상비구유쾌속、은정、특이성고적특점,비상괄합공업화측시。재첨화요함소가와함진22정구,대찬공、부면이급구역소채집적미생물토양양품진행검측연구,결과현시,이용기인정량기술검측도적유기미생물이상여유기전유겁호적대응관계,증명발전유기미생물분자감탐기술기술구유엄활적원경。
Compared with traditional culture-dependent methods, culture-independent techniques based on functional genes for detec-ting oil and gas indicating microorganisms are more rapid, stable and specific, thereby suitable for commercial applications. In this study, the authors chose Chen 22 wells zone in Shaojia subsag of Zhanhua SAG as a model system. Based on the analyses of the soil samples collected from point, linear and areal surveys, the authors detected that the anomalies of gene abundance were perfectly con-sistent with the presence of the oil and gas reservoirs. Therefore, microbial molecular prospecting technique for oil and gas is very prom-ising in the future.