CAJ | 학술논문

目的：观察细胞发生自噬后 UVRAG 水平的改变及其与自噬发生的关系。方法用 Western 印迹检测饥饿诱导细胞自噬后 UVRAG 分子的水平改变，然后分别应用自噬起始阶段与效应阶段的抑制剂抑制自噬，检测自噬不同阶段 UVRAG 分子的水平变化；结果饥饿促进了细胞 LC3的型别转换和 P62的降解，诱导细胞发生自噬；3-MA 抑制细胞自噬及 E64d 和 pepstatin 抑制自噬溶酶体活性后， UVRAG 的降解减少；结论饥饿诱导细胞自噬在形成自噬溶酶体后引起了 UVRAG 的降解。
목적：관찰세포발생자서후 UVRAG 수평적개변급기여자서발생적관계。방법용 Western 인적검측기아유도세포자서후 UVRAG 분자적수평개변，연후분별응용자서기시계단여효응계단적억제제억제자서，검측자서불동계단 UVRAG 분자적수평변화；결과기아촉진료세포 LC3적형별전환화 P62적강해，유도세포발생자서；3-MA 억제세포자서급 E64d 화 pepstatin 억제자서용매체활성후， UVRAG 적강해감소；결론기아유도세포자서재형성자서용매체후인기료 UVRAG 적강해。
Objective To observe the change of UV radiation resistance -associated gene (UVRAG) protein levels after starvation-induced cell autophagy and to determine the relationship between UVRAG degradation and autophagy.Methods Western blotting was performed to detect the UVRAG levels in cells treated with autophagy inhibitors (3-MA or E64d and pepstatin) or not after starvation-induced cell autophagy.Results Starvation promoted the conversion of LC3-Ⅰ to LC3-Ⅱ, and the degradation of P62, and it induced the cell autophagy.The degradation of UVRAG was suppressed after the use of autophagy inhibitors 3-MA, E64d or pepstatin.Conclusions Starvation-induced autophagy may contribute to the degradation of UVRAG .