CAJ | 학술논문

目的：采用TK基因突变试验评价土荆芥精油的遗传毒性，为其安全性评价提供资料。方法：土荆芥精油浓度设置分别为体积分数0.002%、0.004%、0.006%和0.008%，对L5178Y细胞进行3 h染毒处理；同时分别设二甲基亚砜(DMSO)和环磷酰胺(CP)为溶剂对照和阳性对照，微孔板法进行TK基因突变试验。检测土荆芥精油对L5178Y细胞的细胞毒性及TK基因位点突变频率。结果：随土荆芥精油浓度的增大，L5178Y细胞相对存活率(RS)、相对悬浮生长率(RSG)和相对总生长率(RTG)较溶剂对照组有下降趋势；0.008%剂量组时，RS、RSG和RTG分别为溶剂对照组的29.38%、38.13%和5.75%，表现出明显的细胞毒性；与溶剂对照组相比，突变频率(TMF)随土荆芥精油浓度增加则呈上升趋势(F=410.5，P=0.00)。TMF在土荆芥精油各浓度组间以及和阳性、溶剂对照组间的多重比较差异均有统计学意义(P<0.05)。小集落百分比(SC)均超过95%。结论：土荆芥精油具有一定的细胞毒性，可诱导L5178Y细胞TK基因位点突变频率增高，有一定的致突变性。
목적：채용TK기인돌변시험평개토형개정유적유전독성，위기안전성평개제공자료。방법：토형개정유농도설치분별위체적분수0.002%、0.004%、0.006%화0.008%，대L5178Y세포진행3 h염독처리；동시분별설이갑기아풍(DMSO)화배린선알(CP)위용제대조화양성대조，미공판법진행TK기인돌변시험。검측토형개정유대L5178Y세포적세포독성급TK기인위점돌변빈솔。결과：수토형개정유농도적증대，L5178Y세포상대존활솔(RS)、상대현부생장솔(RSG)화상대총생장솔(RTG)교용제대조조유하강추세；0.008%제량조시，RS、RSG화RTG분별위용제대조조적29.38%、38.13%화5.75%，표현출명현적세포독성；여용제대조조상비，돌변빈솔(TMF)수토형개정유농도증가칙정상승추세(F=410.5，P=0.00)。TMF재토형개정유각농도조간이급화양성、용제대조조간적다중비교차이균유통계학의의(P<0.05)。소집락백분비(SC)균초과95%。결론：토형개정유구유일정적세포독성，가유도L5178Y세포TK기인위점돌변빈솔증고，유일정적치돌변성。
OBJECTIVE:The genotoxicity of essential oil fromChenopodium ambrosioides L. was evaluated withTK gene mutation test to provide information for its safety. METHODS:The mouse lymphoma cells L5178Y were exposed to essential oil ofC. ambrosioides at volume fraction concentrations of 0.002%, 0.004%,0.006% and 0.008% for 3 h. 1% dimethyl sulfoxide (DMSO) and 10μg/mL cyclophosphamide (CP) were used as solvent and positive controls,respectively.TK gene mutation was assessed with microwell method to detect cytotoxicity and mutation frequency atTK locus in L5178Y cells.RESULTS:Relative survival (RS),relative suspension growth (RSG) and relative total growth (RTG) decreased when concentration of essential oil fromC. ambrosioides increased,with only 29.38%,38.13% and 5.75%,respectively,in 0.008% dose group indicating significant cytotoxicity. Compared with the solvent control mutation frequency (TMF) increased with the rising concentration of essential oil(F=410.5,P=0.00). Multiple comparisons of TMT between the different essential oil groups,positive control group and solvent control group were statistically significant differences (P<0.05). The percentage of small growth mutant (SCM) was more than 95%. CONCLUSION: Essential oil fromC. ambrosioides showed strong cytotoxicity and could increase mutation frequency at TK gene locus in L5178Y cells.