邻苯二甲酸二（2-乙基己）酯对大鼠卵巢病理和超微结构的影响
린분이갑산이（2-을기기）지대대서란소병리화초미결구적영향
Pathological damage and ultrastructure changes in female rat ovary induced by di(2-ethylhexyl) phthalate
  • 万方数据
    癌变·畸变·突变 암변·기변·돌변
    CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
    2014年 5期 369-373 ,共5页

    谭琴%秦逍云%徐新云%张倩%马佺%杨细飞%吴德生%蒋灵芝%周丽%刘建军%黄海燕%杨淋清%黄新凤%黄冠琴

    담금%진소운%서신운%장천%마전%양세비%오덕생%장령지%주려%류건군%황해연%양림청%황신봉%황관금

    邻苯二甲酸二(2-乙基己)酯%卵巢%卵泡%颗粒细胞%超微结构%生殖毒性 鄰苯二甲痠二(2-乙基己)酯%卵巢%卵泡%顆粒細胞%超微結構%生殖毒性
    린분이갑산이(2-을기기)지%란소%란포%과립세포%초미결구%생식독성
    di(2-ethylhexyl) phthalate%ovary%ovarian follicles%granulose cells%ultrastructure%reproductive toxicity
    目的：探讨邻苯二甲酸二(2-乙基己)酯(DEHP)对大鼠卵巢病理和超微结构的影响，评价DEHP对雌性大鼠的生殖毒性作用。方法：SPF级5周龄雌性SD大鼠40只，随机分为对照组(玉米油)、DEHP低剂量组(100 mg/kg)、DEHP中剂量组(500 mg/kg)和DEHP高剂量组(1500 mg/kg)，每组10只，每天灌胃1次，每周5次，连续染毒6周。染毒结束后称量大鼠体质量及卵巢质量，计算卵巢脏器系数；光镜下观察大鼠卵巢病理改变；电镜观察卵巢颗粒细胞超微结构的变化。结果：各染毒组大鼠体质量与对照组比较差异均无统计学意义(P均>0.05)，高剂量组卵巢质量和脏器系数较对照组明显增加(<0.01)；普通光学显微镜检查发现DEHP处理后卵巢组织卵泡结构受损，电镜观察发现DEHP处理后颗粒细胞线粒体肿胀、线粒体嵴减少、胞浆空泡化、核染色质完全凝聚、出现凋亡小体、细胞变性坏死。结论：DEHP对大鼠卵巢毒性明显，影响卵泡发育，引起颗粒细胞凋亡。P
    목적：탐토린분이갑산이(2-을기기)지(DEHP)대대서란소병리화초미결구적영향，평개DEHP대자성대서적생식독성작용。방법：SPF급5주령자성SD대서40지，수궤분위대조조(옥미유)、DEHP저제량조(100 mg/kg)、DEHP중제량조(500 mg/kg)화DEHP고제량조(1500 mg/kg)，매조10지，매천관위1차，매주5차，련속염독6주。염독결속후칭량대서체질량급란소질량，계산란소장기계수；광경하관찰대서란소병리개변；전경관찰란소과립세포초미결구적변화。결과：각염독조대서체질량여대조조비교차이균무통계학의의(P균>0.05)，고제량조란소질량화장기계수교대조조명현증가(<0.01)；보통광학현미경검사발현DEHP처리후란소조직란포결구수손，전경관찰발현DEHP처리후과립세포선립체종창、선립체척감소、포장공포화、핵염색질완전응취、출현조망소체、세포변성배사。결론：DEHP대대서란소독성명현，영향란포발육，인기과립세포조망。P
    OBJECTIVE: To explore the pathological damage and ultrastructure changes in female rat ovary when rats were treated with di(2-ethylhexyl) phthalate (DEHP),to assess the toxicity of DEHP in female reproduction.METHODS:40 female SD rats were randomly divided into control group (corn oil),DEHP low dose group (100 mg/kg),DEHP medium dose group (500 mg/kg ),DEHP high dose group (1 500 mg/kg). The rats were treated with DEHP by gavage for 6 weeks. Rat body weight and ovary wet weight and ovary coefficients were calculated,the ovarian pathology was studied after HE staining,the ultrastructure changes in granulose cells were examined through electron microscopy.RESULTS:The ovarian weight and ovary coefficients were increased in DEHP high dose group. Pathological changes showed ovarian follicle damage,loose granular cell structure, follicular atresia after DEHP treatment. We also found mitochondria swollen, mitochondrial cristae decreased, cytoplasmic vacuolation, nuclear chromatin condensation,apoptotic bodies present,cell degeneration and necrosis in DEHP treated groups.CONCLUSION:DEHP could affect follicular development,induce granulose cell apoptosis in female rats,and subsequently causing reproductive toxicity.
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