癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2014年
5期
365-368
,共4页
秦逍云%谭琴%徐新云%吴德生%黄海燕
秦逍雲%譚琴%徐新雲%吳德生%黃海燕
진소운%담금%서신운%오덕생%황해연
邻苯二甲酸二(2-乙基己)酯%类固醇快速调节蛋白%3β-羟基类固醇脱氢酶%17-羟基类固醇脱氢酶%促性腺激素释放激素%雌激素受体%基因表达β
鄰苯二甲痠二(2-乙基己)酯%類固醇快速調節蛋白%3β-羥基類固醇脫氫酶%17-羥基類固醇脫氫酶%促性腺激素釋放激素%雌激素受體%基因錶達β
린분이갑산이(2-을기기)지%류고순쾌속조절단백%3β-간기류고순탈경매%17-간기류고순탈경매%촉성선격소석방격소%자격소수체%기인표체β
d i(2-ethylhexyl) p hthalate%steroidogenic a cute r egulatory p rotein%3β-hydroxysteroid d ehydrogenase%17β-hydroxysteroid d ehydrogenase%gonadotropin r eleasing h ormone%estrogen r eceptorα%estrogen r eceptorβ%gene e xpression
目的:探讨邻苯二甲酸二(2-乙基己)酯(DEHP)对雄性大鼠睾丸中激素相关基因表达水平的影响,为研究DEHP生殖毒性提供科学依据。方法:40只5周龄雄性SPF级SD大鼠,随机分为对照组(玉米油)、DEHP低剂量组(100 mg/kg)、DEHP中剂量组(500 mg/kg)和DEHP高剂量组(1500 m g/kg),每组10只,每天灌胃染毒1次,每周5次,连续染毒6周。于末次染毒24 h 后处死动物,实时荧光定量PCR法测定睾丸组织内类固醇快速调节蛋白(StAR)、3β-羟基类固醇脱氢酶(3-HSD)、17-羟基类固醇脱氢酶(17-HSD)、促性腺激素释放激素(GnRH)及雌激素受体(ERα、ERβ)的基因表达水平。结果:与对照组比较,、β基因表达水平在DEHP各剂量组均明显升高,差异均具有统计学意义(P<0.01或P<0.05);17β-HSD基因表达水平在DEHP高剂量组升高(P<0.01),而低剂量组和中剂量组则无明显改变(P>0.05);ERα基因表达在DEHP高剂量组升高(P<0.01);ERβ基因表达在DEHP低剂量组和中剂量组下降(P<0.01),但在DEHP高剂量组升高(P<0.01);GnRH基因表达水平在DEHP中剂量组和高剂量组均显著下降,差异具有统计学意义(P<0.01或P<0.05)。结论:DEHP染毒可引起雄性大鼠激素相关基因表达水平改变,干扰雄性激素的合成,可能导致生殖障碍。βββStAR 3-HSD
目的:探討鄰苯二甲痠二(2-乙基己)酯(DEHP)對雄性大鼠睪汍中激素相關基因錶達水平的影響,為研究DEHP生殖毒性提供科學依據。方法:40隻5週齡雄性SPF級SD大鼠,隨機分為對照組(玉米油)、DEHP低劑量組(100 mg/kg)、DEHP中劑量組(500 mg/kg)和DEHP高劑量組(1500 m g/kg),每組10隻,每天灌胃染毒1次,每週5次,連續染毒6週。于末次染毒24 h 後處死動物,實時熒光定量PCR法測定睪汍組織內類固醇快速調節蛋白(StAR)、3β-羥基類固醇脫氫酶(3-HSD)、17-羥基類固醇脫氫酶(17-HSD)、促性腺激素釋放激素(GnRH)及雌激素受體(ERα、ERβ)的基因錶達水平。結果:與對照組比較,、β基因錶達水平在DEHP各劑量組均明顯升高,差異均具有統計學意義(P<0.01或P<0.05);17β-HSD基因錶達水平在DEHP高劑量組升高(P<0.01),而低劑量組和中劑量組則無明顯改變(P>0.05);ERα基因錶達在DEHP高劑量組升高(P<0.01);ERβ基因錶達在DEHP低劑量組和中劑量組下降(P<0.01),但在DEHP高劑量組升高(P<0.01);GnRH基因錶達水平在DEHP中劑量組和高劑量組均顯著下降,差異具有統計學意義(P<0.01或P<0.05)。結論:DEHP染毒可引起雄性大鼠激素相關基因錶達水平改變,榦擾雄性激素的閤成,可能導緻生殖障礙。βββStAR 3-HSD
목적:탐토린분이갑산이(2-을기기)지(DEHP)대웅성대서고환중격소상관기인표체수평적영향,위연구DEHP생식독성제공과학의거。방법:40지5주령웅성SPF급SD대서,수궤분위대조조(옥미유)、DEHP저제량조(100 mg/kg)、DEHP중제량조(500 mg/kg)화DEHP고제량조(1500 m g/kg),매조10지,매천관위염독1차,매주5차,련속염독6주。우말차염독24 h 후처사동물,실시형광정량PCR법측정고환조직내류고순쾌속조절단백(StAR)、3β-간기류고순탈경매(3-HSD)、17-간기류고순탈경매(17-HSD)、촉성선격소석방격소(GnRH)급자격소수체(ERα、ERβ)적기인표체수평。결과:여대조조비교,、β기인표체수평재DEHP각제량조균명현승고,차이균구유통계학의의(P<0.01혹P<0.05);17β-HSD기인표체수평재DEHP고제량조승고(P<0.01),이저제량조화중제량조칙무명현개변(P>0.05);ERα기인표체재DEHP고제량조승고(P<0.01);ERβ기인표체재DEHP저제량조화중제량조하강(P<0.01),단재DEHP고제량조승고(P<0.01);GnRH기인표체수평재DEHP중제량조화고제량조균현저하강,차이구유통계학의의(P<0.01혹P<0.05)。결론:DEHP염독가인기웅성대서격소상관기인표체수평개변,간우웅성격소적합성,가능도치생식장애。βββStAR 3-HSD
OBJECTIVE: To explore the effects of di(2-ethylhexyl) phthalate (DEHP) on hormonal-related gene expression levels in male rats.METHODS:A total of 40 healthy 5-week-old male Sprague-Dawley rats were randomly divided into four groups with 10 rats in each group. The DEHP doses were 0 mg/kg in control group(corn oil),100 mg/kg in low dose group,500 mg/kg in medium dose group and 1 500 mg/kg in high dose group. Rats were exposed to DEHP via gavage administration for six consecutive weeks,DEHP administration was once a day and five times a week. After exposure,rats were anesthetized by ether,then sacrificed for further study. The relative gene expression levels of steroidogenic acute regulatory protein (StAR),3β-hydroxysteroid dehydrogenase(3β-HSD),17β-hydroxysteroid dehydrogenase (17β-HSD),estrogen receptors (ERα/β) and gonadotropin releasing hormone (GnRH) were analyzed by real time quantitative RT-PCR.RESULTS:Compared with control group,the relative gene expression levels of StAR and 3β-HSD were increased in medium DEHP dose and high DEHP dose groups (P<0.01 orP<0.05). The relative gene expression levels of 17β-HSD,ERα andERβ were increased in high DEHP dose group (P<0.01 orP<0.05),while the relative gene expression levels of GnRH was decreased in medium DEHP dose and high DEHP dose groups(P<0.01 or P<0.05).CONCLUSION:DEHP could cause endocrine disorder and interfere with the synthesis of male hormones in rats,ultimately leading to male reproductive dysfunction.