CAJ | 학술논문

目的：初步探索应用人胚胎干细胞来源的肝细胞作为乙肝病毒(HBV)体外研究模型的潜力。方法：定向分化人胚胎干细胞为肝细胞(hESC-Heps)；采用免疫荧光方法检测肝性因子如肝细胞核因子-4α(HNF4α)、白蛋白(ALB)和HBV功能性受体钠离子/牛磺胆酸共转运蛋白(NTCP)受体的表达；实时荧光定量PCR检测Ⅲ型干扰素受体表达，Western blotting 检测hESC-Heps在Ⅲ型干扰素刺激下干扰素通路中磷酸化转录信号传导子与激活子2(STAT2)的表达。结果：成熟的hESC-Heps表达肝性因子HNF4α和ALB，表达HBV功能性受体NTCP受体，并且具有人原代肝细胞特异性的Ⅲ型干扰素反应。结论：人胚胎干细胞来源的肝细胞hESC-Heps可能成为一种具有潜力的HBV研究模型。
목적：초보탐색응용인배태간세포래원적간세포작위을간병독(HBV)체외연구모형적잠력。방법：정향분화인배태간세포위간세포(hESC-Heps)；채용면역형광방법검측간성인자여간세포핵인자-4α(HNF4α)、백단백(ALB)화HBV공능성수체납리자/우광담산공전운단백(NTCP)수체적표체；실시형광정량PCR검측Ⅲ형간우소수체표체，Western blotting 검측hESC-Heps재Ⅲ형간우소자격하간우소통로중린산화전록신호전도자여격활자2(STAT2)적표체。결과：성숙적hESC-Heps표체간성인자HNF4α화ALB，표체HBV공능성수체NTCP수체，병차구유인원대간세포특이성적Ⅲ형간우소반응。결론：인배태간세포래원적간세포hESC-Heps가능성위일충구유잠력적HBV연구모형。
OBJECTIVE: To explore the potential of human embryonic stem cell-derived hepatocytes (hESC-Heps) for hepatitis Bvirus ( HBV) model.METHODS:Human embryonics temcells were directly induced to differentiate into hESC-Heps using a step-wised method. Expression of hepatocyte-enriched factors such as hepatocyte nuclear factor 4 alpha+(HNF4α) and albumin (ALB),and HBV functional receptor Na/taurocholate cotransporting polypeptide (NTCP) receptor in hESC-Heps were examined by immunofluorescence method. Moreover,activation of signal transducer and activator of transcription 2 (STAT2) in hESC-Heps treated with typeⅢ interferon was examined by Western blotting.RESULTS:Mature hESC-Heps expressed HNF4α,ALB,and NTCP receptor as well as displayed typeⅢ interferon responsespecific in primary human hepatocytes.CONCLUSION:hESC-Heps maybe a potential model for HBV research.