CAJ | 학술논문

报道了微波密闭消解正交试验条件优化电感耦合等离子体发射光谱法(ICP-AES),同时测定8种海洋生物体中硒、铁、锰、锌、钙、镁、锶、砷、镉、铬、铜、铅和镍等13种元素含量的实验结果。优化后的样品消解条件为加入的VHNO3： H2O2V =6.0 mL：1.0 mL,第二步程序升温温度为170℃,消解时间20 min；测定标准曲线相关系数大于0.999,相对标准偏差为0.30%~2.55%,加标回收率为92.0%~104.8%,方法检出限为0.0010~0.0468μg/g。结果表明,优化后方法测定操作简便、具有高的灵敏度、准确度和精密度,且能多元素同时测定,适合于海洋生物样品中这些元素含量的准确测定,并可为评估海产品食用安全提供科学依据。
보도료미파밀폐소해정교시험조건우화전감우합등리자체발사광보법(ICP-AES),동시측정8충해양생물체중서、철、맹、자、개、미、송、신、력、락、동、연화얼등13충원소함량적실험결과。우화후적양품소해조건위가입적VHNO3： H2O2V =6.0 mL：1.0 mL,제이보정서승온온도위170℃,소해시간20 min；측정표준곡선상관계수대우0.999,상대표준편차위0.30%~2.55%,가표회수솔위92.0%~104.8%,방법검출한위0.0010~0.0468μg/g。결과표명,우화후방법측정조작간편、구유고적령민도、준학도화정밀도,차능다원소동시측정,괄합우해양생물양품중저사원소함량적준학측정,병가위평고해산품식용안전제공과학의거。
Herein, thirteen elements including Se, Fe, Mn, Zn, Ca, Mg, Sr, As, Cd, Cr, Cu, Pb and Ni in eight kinds of marine organisms were determined by using microwave digestion technique, and applying Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES). The conditions of microwave digestion were optimized through orthogonal experiment design. The optimum conditions were as follows: the volume ratio of HNO3-H2O2︰6︰1, digestion temperature: 170 ℃, and digestion time: 20 min. The correlation coefficients of linear regression equation for all elements were above 0.999. The recovery rate of the method was in the range of 92.0%~104.8%, and the relative standard deviations were between 0.30%and 2.55%. The detection limit of the method ranged from 0.0010μg/g to 0.0468μg/g. The results indicated that the optimized method was convent, sensitivity, accurate and precision, and also can be used for simultaneous multi-element detection. It was suitable for determination of these elements in marine organisms, and also provided scientific foundation for evaluation of safety of seafood.