CAJ | 학술논문

从红树植物红海榄叶片中分离一株对芘具有较好降解作用的海洋细菌,命名为 B11,并对其菌体特征、生长条件及降解效能等进行了系统研究。结果发现该菌株为革兰氏阴性菌,结合其生理生化特征和16S rDNA序列比对,表明此菌株属于交替单胞菌属(Alteromonas sp.)。菌株在芘的起始浓度为100 mg/L, pH为中性或偏碱性条件下,盐度35时对芘的降解作用最强。外加碳源对B11对芘的降解效果都具有一定的抑制作用,其中水杨酸的抑制作用更为显著。综合菌株B11的生长特性和降解效能,初步认为B11菌株较适合用于降解多环芳烃芘,可用于红树植物多环芳烃生态污染的原位修复。
종홍수식물홍해람협편중분리일주대비구유교호강해작용적해양세균,명명위 B11,병대기균체특정、생장조건급강해효능등진행료계통연구。결과발현해균주위혁란씨음성균,결합기생리생화특정화16S rDNA서렬비대,표명차균주속우교체단포균속(Alteromonas sp.)。균주재비적기시농도위100 mg/L, pH위중성혹편감성조건하,염도35시대비적강해작용최강。외가탄원대B11대비적강해효과도구유일정적억제작용,기중수양산적억제작용경위현저。종합균주B11적생장특성화강해효능,초보인위B11균주교괄합용우강해다배방경비,가용우홍수식물다배방경생태오염적원위수복。
A predominant indigenous bacterium utilizing pyrene as a sole carbon and energy source was isolated from mangrove (Rhizophora stylosa) in Zhanjiang, China. The morphology, physiological and biochemcial characteristics, capacity of degradation were studied. The results showed that the strain was Gram-negative bacteria . Based on its characteristics mentioned above as well as the 16 S rDNA sequence analysis, the bacterium was identified as Alteromonas sp., and named B11. The best pyrene degradation condition of B11 was that the initial concentration of pyrene was 100 mg/L, pH was 7 or a little alkalinity and salinity was almost 35. Extra carbon source especially salicylic acid reduced degradation rate of pyrene by B11 strain. The results showed that strain B11 was suitable to degrade Pyrene and indigenous rehabilitation in mangrove polluted zone.