CAJ | 학술논문

目的：探讨纳米二氧化钛灌胃染毒对幼年大鼠骨髓细胞的遗传损伤作用。方法将28只清洁级雄性SD幼年大鼠（4周龄），按体重以随机数字表法分为4组（每组7只），每天1次灌胃，分别给予0、10、50、200 mg/kg纳米二氧化钛[（75±15） nm，锐钛矿]，染毒30 d后取骨髓细胞涂片，进行微核试验和γ-H2 AX免疫荧光检测。结果50 mg/kg纳米二氧化钛染毒组大鼠的γ-H2 AX焦点阳性细胞率[（37.4±10.0）％]，明显高于对照组[（19.8±3.1）％]，差异有统计学意义（ t ＝-17.59，P＜0.01）。各染毒组大鼠与对照组相比，骨髓嗜多染红细胞与正染红细胞的比值（ PCE/NCE）和嗜多染红细胞微核率无明显变化。结论短期经口摄入纳米二氧化钛，可导致幼年大鼠骨髓细胞DNA双链断裂增加，但对骨髓细胞微核无明显影响。
목적：탐토납미이양화태관위염독대유년대서골수세포적유전손상작용。방법장28지청길급웅성SD유년대서（4주령），안체중이수궤수자표법분위4조（매조7지），매천1차관위，분별급여0、10、50、200 mg/kg납미이양화태[（75±15） nm，예태광]，염독30 d후취골수세포도편，진행미핵시험화γ-H2 AX면역형광검측。결과50 mg/kg납미이양화태염독조대서적γ-H2 AX초점양성세포솔[（37.4±10.0）％]，명현고우대조조[（19.8±3.1）％]，차이유통계학의의（ t ＝-17.59，P＜0.01）。각염독조대서여대조조상비，골수기다염홍세포여정염홍세포적비치（ PCE/NCE）화기다염홍세포미핵솔무명현변화。결론단기경구섭입납미이양화태，가도치유년대서골수세포DNA쌍련단렬증가，단대골수세포미핵무명현영향。
Objective To explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.Methods Twenty-eight SD male young rats ( 4 weeks old ) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75 ±15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight ( bw) every day for 30 days.The bone marrow cells were collected for micronuclei and γ-H2 AX immunofluorescence analysis.Results The percentage of γ-H2 AX foci-positive cells (37.4 ±10.0)%in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8 ±3.1)% (t value was -17.59, P<0.01).No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte ( PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.Conclusion TiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells , but has no effect on micronucleus of bone marrow cells in young rats.