CAJ | 학술논문

目的：探讨SALL4在调节小细胞肺癌多药耐药中的作用，并分析其表达与临床预后的关系。方法采用即时荧光定量PCR法及Western blot 检测小细胞肺癌敏感细胞株H69、耐药细胞株H69AR中SALL4的表达，应用小干扰RNA抑制耐药细胞株H69AR中的SALL4的表达，通过细胞增殖检测试剂盒检测细胞对各种化疗药物（顺铂、多柔比星、依托泊苷）的敏感性；免疫组织化学方法检测小细胞肺癌患者组织标本中SALL4的表达，分析SALL4与小细胞肺癌患者预后相关性。结果SALL4在H69AR细胞中的表达明显高于H69细胞，差异具有统计学意义（P＜0.01）；通过小干扰RNA抑制耐药细胞株H69AR中SALL4的表达能够增加细胞对化疗药物的敏感性，差异具有统计学意义（P＝0.02）；SALL4在小细胞肺癌肿瘤组织中的表达较癌旁组织明显增高，差异具有统计学意义（P＜0.01）。 SALL4的表达与患者的性别、年龄无关，与疾病的分期、对化疗的敏感性及生存时间密切相关，差异具有统计学意义（ P＜0.05）。结论 SALL4参与了调节小细胞肺癌的多药耐药，SALL4可作为评估小细胞肺癌化疗敏感性及临床预后的潜在靶基因。
목적：탐토SALL4재조절소세포폐암다약내약중적작용，병분석기표체여림상예후적관계。방법채용즉시형광정량PCR법급Western blot 검측소세포폐암민감세포주H69、내약세포주H69AR중SALL4적표체，응용소간우RNA억제내약세포주H69AR중적SALL4적표체，통과세포증식검측시제합검측세포대각충화료약물（순박、다유비성、의탁박감）적민감성；면역조직화학방법검측소세포폐암환자조직표본중SALL4적표체，분석SALL4여소세포폐암환자예후상관성。결과SALL4재H69AR세포중적표체명현고우H69세포，차이구유통계학의의（P＜0.01）；통과소간우RNA억제내약세포주H69AR중SALL4적표체능구증가세포대화료약물적민감성，차이구유통계학의의（P＝0.02）；SALL4재소세포폐암종류조직중적표체교암방조직명현증고，차이구유통계학의의（P＜0.01）。 SALL4적표체여환자적성별、년령무관，여질병적분기、대화료적민감성급생존시간밀절상관，차이구유통계학의의（ P＜0.05）。결론 SALL4삼여료조절소세포폐암적다약내약，SALL4가작위평고소세포폐암화료민감성급림상예후적잠재파기인。
Objective To investigate the role of SALL 4 in regulating multi-drug resistance in small cell lung cancer ( SCLC), and to evaluate its clinical significance .Methods The expression of SALL4 protein and gene was detected by Western blot and real-time PCR (RT-PCR) in both H69 and H69AR cell lines, respectively.SALL4 expression in H69AR was blocked by the siRNA, and then the drug-sensitivities of H69AR cell lines to chemotherapeutic drugs such as cisplatin , doxorubicin, and etoposide were evaluated by cell counting kit assay .SALL4 expression was also examined by immunohistochemistry , and correlated with patients′clinicopathological features and prognosis . Results The expression of SALL4 was significantly increased in H69AR cells than in the H69 cells ( P <0.01).Down-regulation of SALL4 increased the drug-sensitivities of H69AR cells to chemotherapeutic drugs (P=0.02).The expression of SALL4 was significantly increased in SCLC than in para-carcinoma tissues ( P<0.01 ) .SALL4 expression correlated with clinical stage , chemosensitivity and overall survival ( P<0.05 ) , but not with patients′age and gender .Conclusion SALL4 is involved in the regulation of multidrug resistance in SCLC;SALL4 may be a potential target gene to evaluate the chemosensitivity and clinical prognosis for SCLC .