CAJ | 학술논문

为获得含有天花粉蛋白（ TCS）的转基因欧美杨108植株，进行了植物表达载体的构建以及导入欧美杨108的研究。将北京大学惠赠的含有TCS启动子及结构基因的质粒pT1-3经SacⅠ和BamHⅠ双酶切后与同样双酶切的载体pCambia1301用T4 DNA连接酶相连，转化大肠杆菌，构建植物表达载体pC-tPro-TCS。将构建的植物表达载体转入农杆菌LBA4404，采用农杆菌介导法，将其导入欧美杨108中。结果表明，共得到18个潮霉素抗性愈伤和抗性芽，转化效率为4．3％。抗性体经PCR和PCR-Southern检测，初步确认TCS基因已经导入到欧美杨108中。
위획득함유천화분단백（ TCS）적전기인구미양108식주，진행료식물표체재체적구건이급도입구미양108적연구。장북경대학혜증적함유TCS계동자급결구기인적질립pT1-3경SacⅠ화BamHⅠ쌍매절후여동양쌍매절적재체pCambia1301용T4 DNA련접매상련，전화대장간균，구건식물표체재체pC-tPro-TCS。장구건적식물표체재체전입농간균LBA4404，채용농간균개도법，장기도입구미양108중。결과표명，공득도18개조매소항성유상화항성아，전화효솔위4．3％。항성체경PCR화PCR-Southern검측，초보학인TCS기인이경도입도구미양108중。
In order to obtain transgenic Populus euramericana with Trichosanthin ( TCS) gene, a plant expression vector was constructed and TCS gene was transformed into P.euramericana.The plasmid pT1-3 containing TCS promoter and structur-al gene which was donated by Peking University was digested by SacⅠand BamHⅠ, the vector pCambia1301 was digested by the same enzymes.The digested and purified TCS genes were cloned into plant expression vector pC-tPro-TCS.The pC-tPro-TCS with TCS gene was successfully transferred into Agrobacterium tumefaciens LBA4404, adapting to Agrobacterium-mediated to transform the leaf explants, and 18 resistant shoots were obtained with transformation frequency of 4.3%.The resistant shoots were identified by PCR and PCR-Southern analysis.TCS gene was transferred into P.euramericana 108.