CAJ | 학술논문

以梅花鹿茸为原料，采用超声波辅助法提取可溶性蛋白。以鹿茸可溶性蛋白得率为评价指标，通过单因素试验研究提取溶剂浓度、超声提取时间、超声提取功率和液料比对鹿茸可溶性蛋白得率的影响，在单因素试验的基础上进行正交试验，优化提取工艺条件。结果表明：鹿茸可溶性蛋白的最优提取工艺条件为，以pH＝10．00、0．05 mol· L-1的碳酸盐缓冲溶液为提取溶剂，液料比10∶1，超声提取功率200 W，超声提取时间20 min。在此条件下，鹿茸可溶性蛋白得率为36．849 mg· g-1。体内免疫活性试验表明，鹿茸蛋白提取物能够提高免疫功能低下小鼠的单核巨噬细胞吞噬指数、血清溶菌酶质量浓度、血清及肝组织中酸性磷酸酶活力，具有良好的免疫活性。
이매화록용위원료，채용초성파보조법제취가용성단백。이록용가용성단백득솔위평개지표，통과단인소시험연구제취용제농도、초성제취시간、초성제취공솔화액료비대록용가용성단백득솔적영향，재단인소시험적기출상진행정교시험，우화제취공예조건。결과표명：록용가용성단백적최우제취공예조건위，이pH＝10．00、0．05 mol· L-1적탄산염완충용액위제취용제，액료비10∶1，초성제취공솔200 W，초성제취시간20 min。재차조건하，록용가용성단백득솔위36．849 mg· g-1。체내면역활성시험표명，록용단백제취물능구제고면역공능저하소서적단핵거서세포탄서지수、혈청용균매질량농도、혈청급간조직중산성린산매활력，구유량호적면역활성。
We extracted the soluble proteins from velvet antler of Cervus nippon Temminck with ultrasonic waves, studied the effect of concentration of extraction solvent, ultrasonic extraction time, ultrasonic extraction power and liquid-material ratio on the velvet antler soluble proteins yield by the single factor experiment, and optimized extraction process conditions by orthogonal experiment.The optimal extraction process conditions of soluble proteins from velvet antler were the extraction solvent of 0.05 mol/L, pH of 10.00, carbonate buffer solution and the liquid-material radio of 10:1, the ultrasonic extrac-tion power of 200 W, and the ultrasonic extraction time of 20 min.The yield of soluble proteins from velvet antler was 36. 849 mg/g.The results of animal experiment on immune activity showed that velvet antler proteins could increase the mono-nuclear macrophage phagocytosis index, the concentration of serum lysozyme, the activity of acid phosphatase in serum and liver tissue of immunosuppressive mice with better immune activity.