CAJ | 학술논문

目的：建立薄层色谱法和高效液相色谱-蒸发光散射检测法（HPLC - ELSD），对清热解毒口服液进行山银花检查。方法清热解毒口服液样品以 D101大孔吸附树脂处理，富集山银花的特征成分灰毡毛忍冬皂苷乙。以该成分为山银花的检出指标，采用 TLC 法进行样品的初筛，并以 HPLC - ELSD 法进行限量检查。结果两个厂家提供的样品中检出山银花。结论建立的方法简便、准确，可用于判断清热解毒口服液中金银花的投料情况。
목적：건립박층색보법화고효액상색보-증발광산사검측법（HPLC - ELSD），대청열해독구복액진행산은화검사。방법청열해독구복액양품이 D101대공흡부수지처리，부집산은화적특정성분회전모인동조감을。이해성분위산은화적검출지표，채용 TLC 법진행양품적초사，병이 HPLC - ELSD 법진행한량검사。결과량개엄가제공적양품중검출산은화。결론건립적방법간편、준학，가용우판단청열해독구복액중금은화적투료정황。
Objective To establish TLC and HPLC - ELSD methods for the inspection of Lonicerae flos in Qingre Jiedu Oral Solution. Methods The sample of Qingre Jiedu Oral Solution was chromatographed over a D101 macroporous resin column to enrich macranthoidin B,which was a characteristic component of Lonicerae flos and thus used as the detec-tion index. Preliminary screening of the inspection was carried out with a TLC method;An HPLC - ELSD method was ap-plied to determine the content of Lonicerae flos. Results Lonicerae flos was detected in samples supplied from two enterpri-ses. Conclusion The methods were simple and accurate,which can be applied to detect the use of Lonicerae japonicae flos in Qingre Jiedu Oral Solution.