CAJ | 학술논문

通过组织分离法从野生地黄组织中分离得到24株内生菌，采用2，4-二硝基苯肼显色法对所分离的菌株进行产梓醇性能的测定，筛选得到一株产梓醇性能较好的菌株 BLCC1-0148，研究其10代内培养物产梓醇性能的遗传稳定性，结合菌落形态特征及菌株特异性序列分析等手段，最终将 BLCC1-0148鉴定为蜡样芽孢杆菌（Ba-cillus Cereus）。利用正交设计试验对高产菌株 BLCC1-0148进行发酵条件的优化，最佳发酵培养基如下：葡萄糖0.5%、蛋白胨0.5%、酵母膏0.75%、氯化钠1%；发酵温度37℃，摇床转数180 rpm，发酵时间3 d，梓醇产量达到839μg·mL -1；
통과조직분리법종야생지황조직중분리득도24주내생균，채용2，4-이초기분정현색법대소분리적균주진행산재순성능적측정，사선득도일주산재순성능교호적균주 BLCC1-0148，연구기10대내배양물산재순성능적유전은정성，결합균락형태특정급균주특이성서렬분석등수단，최종장 BLCC1-0148감정위사양아포간균（Ba-cillus Cereus）。이용정교설계시험대고산균주 BLCC1-0148진행발효조건적우화，최가발효배양기여하：포도당0.5%、단백동0.5%、효모고0.75%、록화납1%；발효온도37℃，요상전수180 rpm，발효시간3 d，재순산량체도839μg·mL -1；
24 strains of entophyte were isolated from Rehmannia by tissue isolation. The 2,4 - dinitrobenzene hydrazine chromogenic method were used to determing the performance of catalpa alcohol from separated strains. The strain BLCC1 -0148 with better performance production of catalpol and good genetic stability were screened and had been identified as Ba-cillus Cereus by based on physiological,biochemical characteristics and 16S rDNA sequence phylogeny analysis. The cultur-al conditions of BLCC1 - 0148 strain was optimized by the orthogonal design experiment. The optimum medium composition of fermentation conditions was as follows:glucose 0. 5% ,tryptone 0. 5% ,yeast extract 0. 75% ,NaCl 1% . The fermentation conditions included cultural temperature 37 ℃,rotational speed of rocking bed 180 rpm,fermentation time 3d. The produc-tion of catalpol reached 839 μg·mL - 1.