CAJ | 학술논문

以无患子皂素水提水解液为底物，发酵生产生物表面活性剂，在精制无患子皂素的同时发酵联产生物表面活性剂鼠李糖脂。经铜绿假单胞菌发酵后发酵完成液中不含葡萄糖，葡萄糖的消耗速率与接种量成正相关，在发酵液中额外添加大豆油可促进表面活性剂亲油基团的生成，溶液表面活性进一步提高。发酵后溶液中表面活性物质浓度达到50.8g/L，比对照组提高了16.1%，溶液表面张力明显降低。接种10%的菌种发酵获得的无患子皂素复合产物干粉其临界胶束浓度由10g/L降低到2.5g/L，临界胶束浓度下的复合产物水溶液表面张力比未接种菌种的低18.67%，复合产物具有很好的起泡性能及更高的泡沫稳定性。
이무환자조소수제수해액위저물，발효생산생물표면활성제，재정제무환자조소적동시발효련산생물표면활성제서리당지。경동록가단포균발효후발효완성액중불함포도당，포도당적소모속솔여접충량성정상관，재발효액중액외첨가대두유가촉진표면활성제친유기단적생성，용액표면활성진일보제고。발효후용액중표면활성물질농도체도50.8g/L，비대조조제고료16.1%，용액표면장력명현강저。접충10%적균충발효획득적무환자조소복합산물간분기림계효속농도유10g/L강저도2.5g/L，림계효속농도하적복합산물수용액표면장력비미접충균충적저18.67%，복합산물구유흔호적기포성능급경고적포말은정성。
Sapindus saponin water extraction , hydrolyzed by Cellobiase and inoculated with Pseudomonas aeruginosa,was fermented to produce biosurfactant rhamnolipid. After fermentation, glucose was completely consumed,and glucose consumption rate was positively rated with loading of Pseudomonas aeruginosa. In the fermentation broth,additional soybean oil could promote formation of surfactant lipophilic group,and solution surface activity was further improved. After fermentation,the concentration of surface active material reached 50.8g/L,16.1% higher than that of the control experiment. The surface tension of the solution was significantly decreased. The critical micelle concentration of Sapindus saponin powder composite with 10%inoculum was reduced from 10g/L to 2.5g/L,and surface tension was 18.67%lower than Sapindus saponin powder without inoculum. The composite product had good foaming properties and high foam stability.