CAJ | 학술논문

细胞高密度培养有利于降低微藻规模化培养成本及其生物柴油制造的成本，曝气是影响微藻规模化高密度培养的重要因素之一。以普通小球藻（Chlorella vulgaris，FACHB-1227）为研究对象，采用BG11培养基，于新型套管式沿程曝气光生物反应系统中，以细胞密度为检测指标，实验研究了曝气间隔时间对藻液中细胞密度、藻液pH值、溶氧量变化的影响。控制每次曝气时气体流量为10L/min、持续时间为0.5h，培养周期为15天。结果表明，藻液中积累的溶解氧能够及时排除，进入生物质积累稳定期时，藻液的pH值基本恒定；微藻生长稳定期时（培养12天），曝气间隔0.5h时细胞密度为7.22×106个/mL，相比于1h、1.5h、2h分别提高了9.56%、41.02%和122.1%。可见，适当减少曝气间隔时间，可显著提高藻细胞密度。
세포고밀도배양유리우강저미조규모화배양성본급기생물시유제조적성본，폭기시영향미조규모화고밀도배양적중요인소지일。이보통소구조（Chlorella vulgaris，FACHB-1227）위연구대상，채용BG11배양기，우신형투관식연정폭기광생물반응계통중，이세포밀도위검측지표，실험연구료폭기간격시간대조액중세포밀도、조액pH치、용양량변화적영향。공제매차폭기시기체류량위10L/min、지속시간위0.5h，배양주기위15천。결과표명，조액중적루적용해양능구급시배제，진입생물질적루은정기시，조액적pH치기본항정；미조생장은정기시（배양12천），폭기간격0.5h시세포밀도위7.22×106개/mL，상비우1h、1.5h、2h분별제고료9.56%、41.02%화122.1%。가견，괄당감소폭기간격시간，가현저제고조세포밀도。
High-density cell culture could reduce the cost of large-scale cultivation of microalgae for biodiesel. Aeration is one of the important factors in high-density culture of microalgae. Chlorella vulgaris was cultured for 15 days in a novel concentric-tube photobioreactor with BG11 medium. The effect of different aeration intervals on cell density of Chlorella vulgaris,pH and dissolved oxygen changes of medium was studied. Air was passed into the concentric-tube photobioreactor at aeration flow rate 10L/min for 0.5h each aeration. The aeration manner of the novel concentric-tube photobioreactor could exclude dissolved oxygen timely. When biomass accumulation was stable,the pH of culture medium was substantially constant;the cell density was 7.22×106 cells/mL at aeration interval 0.5h. Compared to the cell concentration at aeration interval 1h , 1.5h , 2h , the cell concentrations at aeration interval 0.5h increased by 9.56%,41.02%,122.1%,respectively. Therefore, with the decrease of aeration interval time,concentration of Chlorella vulgaris increased.