CAJ | 학술논문

搅拌是影响发酵过程的主要因素之一，组合不同类型搅拌桨、发挥其各自优势，势必能够优化搅拌性能、提高微生物发酵生产效率。本研究采用计算流体力学（CFD）方法，模拟六直叶涡轮桨（DT）和DT、DT和抛物线桨（BT6）、六斜叶桨（CM6）和DT以及CM6和BT6四种搅拌桨组合对流场和混合时间的影响。对模拟得到的混合时间进行实验验证。结果表明，最优组合为：上桨叶为 CM6桨，下桨叶为 BT6桨。吸水链霉菌WSH03-13产谷氨酰胺转胺酶（TG）的发酵实验结果表明：CM6和BT6桨组合可获得最高的谷氨酰胺转胺酶活性和生物量，分别为4.7U/mL和42.9g/L，较优化前（DT和DT桨）分别提高了53%和40.9%，说明优化后的搅拌桨组合更有利于微生物生长和发酵。研究结果为组合搅拌桨在微生物发酵过程中的应用提供了借鉴。
교반시영향발효과정적주요인소지일，조합불동류형교반장、발휘기각자우세，세필능구우화교반성능、제고미생물발효생산효솔。본연구채용계산류체역학（CFD）방법，모의륙직협와륜장（DT）화DT、DT화포물선장（BT6）、륙사협장（CM6）화DT이급CM6화BT6사충교반장조합대류장화혼합시간적영향。대모의득도적혼합시간진행실험험증。결과표명，최우조합위：상장협위 CM6장，하장협위 BT6장。흡수련매균WSH03-13산곡안선알전알매（TG）적발효실험결과표명：CM6화BT6장조합가획득최고적곡안선알전알매활성화생물량，분별위4.7U/mL화42.9g/L，교우화전（DT화DT장）분별제고료53%화40.9%，설명우화후적교반장조합경유리우미생물생장화발효。연구결과위조합교반장재미생물발효과정중적응용제공료차감。
Agitation is one of the main factors affecting fermentation process. To take advantage of advantages of individual impellers,impeller combination is necessary in improving agitation and fermentation efficiency. Four different impeller combinations (DT and DT,DT and BT6,CM6 and DT as well as CM6 and BT6) were simulated with the commercial fluid dynamics (CFD) software Fluent and their flow patterns and mixing times were compared. Mixing time was also validated by experiments. The optimal combination was the CM6 (upper impeller) and BT6 (lower impeller). Using this impeller combination , the maximal transglutaminase activity and biomass of Streptomyces hygroscopicus WSH03-13 reached 4.7U/mL and 42.9g/L respectively,which were 53% and 40.9%higher than the control group (DT and DT). The optimized impeller combination could promote microbial growth and fermentation process. The results in this study will be useful for further application of impeller combination in microbial fermentation.