中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
16期
45-46
,共2页
生血宝合剂%制何首乌%2,3,5,4’四羟基二苯乙烯-2-O-β-D-葡萄糖苷%高效液相色谱法
生血寶閤劑%製何首烏%2,3,5,4’四羥基二苯乙烯-2-O-β-D-葡萄糖苷%高效液相色譜法
생혈보합제%제하수오%2,3,5,4’사간기이분을희-2-O-β-D-포도당감%고효액상색보법
Shengxuebao Mixture%prepared radix polygoni multiflori%2,3,5,4’- tetrahydroxy - stilbene - 2 - O - β - D - glycoside%HPLC
目的:建立测定生血宝合剂中君药制何首乌的有效成分二苯乙烯苷(2,3,5,4’-四羟基二苯乙烯-2- O -β- D -葡萄糖苷)含量的高效液相色谱(HPLC)法,为生血宝合剂的质量控制提供依据。方法 Waters alliance 高效液相色谱仪,色谱柱为 Suntek Kromasil C18柱(250 mm ×4.6 mm,5μm),流动相为乙腈-水(20:80),流速为1.0 mL / min,检测波长320 nm。结果二苯乙烯苷在0.05460~1.09200μg 范围内与峰面积积分值线性关系良好( R2=0.9999)。结论该方法灵敏度高、定量准确、方法简便、重现性好,能有效控制生血宝合剂的质量。
目的:建立測定生血寶閤劑中君藥製何首烏的有效成分二苯乙烯苷(2,3,5,4’-四羥基二苯乙烯-2- O -β- D -葡萄糖苷)含量的高效液相色譜(HPLC)法,為生血寶閤劑的質量控製提供依據。方法 Waters alliance 高效液相色譜儀,色譜柱為 Suntek Kromasil C18柱(250 mm ×4.6 mm,5μm),流動相為乙腈-水(20:80),流速為1.0 mL / min,檢測波長320 nm。結果二苯乙烯苷在0.05460~1.09200μg 範圍內與峰麵積積分值線性關繫良好( R2=0.9999)。結論該方法靈敏度高、定量準確、方法簡便、重現性好,能有效控製生血寶閤劑的質量。
목적:건립측정생혈보합제중군약제하수오적유효성분이분을희감(2,3,5,4’-사간기이분을희-2- O -β- D -포도당감)함량적고효액상색보(HPLC)법,위생혈보합제적질량공제제공의거。방법 Waters alliance 고효액상색보의,색보주위 Suntek Kromasil C18주(250 mm ×4.6 mm,5μm),류동상위을정-수(20:80),류속위1.0 mL / min,검측파장320 nm。결과이분을희감재0.05460~1.09200μg 범위내여봉면적적분치선성관계량호( R2=0.9999)。결론해방법령민도고、정량준학、방법간편、중현성호,능유효공제생혈보합제적질량。
Objective To establish a high performance liquid chromatography(HPLC) method for determining the content of 2,3,5, 4’- tetrahydroxy - stilbene - 2 - O - β - D - glycoside as the effective ingredients of the monarch drug prepared radix polygoni multiflori in Shengxuebao Mixture. Methods The Waters alliance high performance liquid chromatograph was adopted with the chromatographic column of the SUNTEK Kromasil C18(250 mm × 4. 6 mm,5 μm). The mobile phase was acetonitrile - water(20 : 80),the flow rate was 1. 0 mL / min and the detected wavelength was 320 nm. Results The sample size of 2,3,5,4’- tetrahydroxy - stilbene - 2 - O - β - D -glycoside in the range of 0. 054 60 - 1. 092 00 μg showed good linear relationship with the peak area integral value( r = 0. 999 9). Conclusion This method is highly sensitive,accurately quantitative,simple and convenient with good reproducibility,and can effectively control the quality of Shengxuebao Mixture.