CAJ | 학술논문

目的：采用高效液相色谱梯度洗脱法测定三七总皂苷中人参皂苷 Rb1、人参皂苷 Rg1和三七皂苷 R1的含量。方法色谱柱为 C18柱（250 mm ×4.6 mm，3.5μm），流动相为乙腈-水、梯度洗脱，检测波长为203nm，流速1.0 mL / min，柱温为室温。结果人参皂苷 Rg1进样量线性范围是6.0~18μg（ r =0.9967），平均回收率为98.79%，RSD =0.33%（ n =6）；人参皂苷 Rb1进样量线性范围是6.0~18μg （ r =0.9964），平均回收率为98.64%，RSD =0.46%（ n =6）；三七皂苷 R1进样量线性范围是1.6~4.8μg（ r =0.9971），平均回收率为98.84%，RSD =0.56%（ n =6）。结论该方法准确、灵敏度高，可用于三七总皂苷的质量控制。
목적：채용고효액상색보제도세탈법측정삼칠총조감중인삼조감 Rb1、인삼조감 Rg1화삼칠조감 R1적함량。방법색보주위 C18주（250 mm ×4.6 mm，3.5μm），류동상위을정-수、제도세탈，검측파장위203nm，류속1.0 mL / min，주온위실온。결과인삼조감 Rg1진양량선성범위시6.0~18μg（ r =0.9967），평균회수솔위98.79%，RSD =0.33%（ n =6）；인삼조감 Rb1진양량선성범위시6.0~18μg （ r =0.9964），평균회수솔위98.64%，RSD =0.46%（ n =6）；삼칠조감 R1진양량선성범위시1.6~4.8μg（ r =0.9971），평균회수솔위98.84%，RSD =0.56%（ n =6）。결론해방법준학、령민도고，가용우삼칠총조감적질량공제。
Objective To establish a HPLC gradient elution method for the determination of ginsenoside Rb1,ginsenoside Rg1and notoginsenoside R1 in Panax notoginseng saponins. Methods The HPLC method was carried out on the C18 column(250 mm × 4. 6 mm, 3. 5 μm) with acetonitrile - water as the mobile phase. The detection wavelength was 203 nm and the flow rate was 1. 0 mL / min.Results The linear range of the ginsenoside Rg1 sample size was 6. 0 - 18 μg( r = 0. 996 7),the average recovery rate was 98. 79% ,RSD = 0. 33%( n = 6). The linear range of ginsenoside Rb1 sample size was 6. 0 - 18 μg( r = 0. 996 4),the average recovery rate was 98. 64% ,RSD = 0. 46%( n = 6). The linear range of notoginsenoside R1 sample size was 1. 6 - 4. 8 μg( r = 0. 997 1),the average recovery rate was 98. 84% ,RSD = 0. 56%( n = 6). Conclusion This method is accurate and highly sensitive,and can be used to control the quality of Panax notoginseng saponins. Key words:panax notoginseng saponins.