CAJ | 학술논문

通过荧光光谱法研究了在维生素C存在下左氧氟沙星对牛血清白蛋白的荧光淬灭作用。在模拟生理条件（ pH＝7.4，37℃）下，根据Stem-Volmer方程，确定了在维生素C存在条件下，左氧氟沙星与牛血清白蛋白的淬灭类型仍为静态淬灭，左氧氟沙星对牛血清白蛋白的荧光淬灭减弱，结合常数和结合位点均变小。为研究左氧氟沙星和维生素对蛋白质构象的影响等提供了重要信息。
통과형광광보법연구료재유생소C존재하좌양불사성대우혈청백단백적형광쉬멸작용。재모의생리조건（ pH＝7.4，37℃）하，근거Stem-Volmer방정，학정료재유생소C존재조건하，좌양불사성여우혈청백단백적쉬멸류형잉위정태쉬멸，좌양불사성대우혈청백단백적형광쉬멸감약，결합상수화결합위점균변소。위연구좌양불사성화유생소대단백질구상적영향등제공료중요신식。
To study the binding interaction of levofloxacin with bovine serum albumin in the presence of Vc by fluorescence spectrophotometry was studied.After analyzing the fluorescence data according to Stem -Volmer equation in physiological condition ( pH=7.4 , 37 ℃) , the type of fluorescence quenching of levofloxacin was static quenching.Vc can decrease the combining constant and binding site of levofloxacin with Bovine Serum Albumin ( BSA ) , and the fluorescence quenching of levofloxacin was reduced with BSA.The results provided important information for the research of levofloxacin and configuration modification of BSA by levofloxacin and vitamin.