广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2014年
17期
124-126
,共3页
廖九明%廖厚知%陈晓军%莫少红%阮碧芳%黄园%陆红萍
廖九明%廖厚知%陳曉軍%莫少紅%阮碧芳%黃園%陸紅萍
료구명%료후지%진효군%막소홍%원벽방%황완%륙홍평
高效液相色谱法%宫炎康颗粒%芍药苷
高效液相色譜法%宮炎康顆粒%芍藥苷
고효액상색보법%궁염강과립%작약감
HPLC%Gongyankang Granule%paeoniflorin
建立了宫炎康颗粒中芍药苷的含量测定方法。采用反相高效液相色谱法， YMC pack ODS-A C18色谱柱（150 mm ×4.6 mm，5μm）；乙腈-0.1％磷酸溶液（12.5：87.5）为流动相；检测波长为230 nm，流速1.0 mL· min-1。结果表明：芍药苷在0.222～2.220μg范围内与峰面积呈良好线性关系， r＝0.9999，芍药苷平均加样回收率为99.19％， RSD为0.56％，精密度、稳定性良好。本方法可靠性高，分离度、准确性、重复性好，适用于宫炎康颗粒中芍药苷的含量测定。
建立瞭宮炎康顆粒中芍藥苷的含量測定方法。採用反相高效液相色譜法， YMC pack ODS-A C18色譜柱（150 mm ×4.6 mm，5μm）；乙腈-0.1％燐痠溶液（12.5：87.5）為流動相；檢測波長為230 nm，流速1.0 mL· min-1。結果錶明：芍藥苷在0.222～2.220μg範圍內與峰麵積呈良好線性關繫， r＝0.9999，芍藥苷平均加樣迴收率為99.19％， RSD為0.56％，精密度、穩定性良好。本方法可靠性高，分離度、準確性、重複性好，適用于宮炎康顆粒中芍藥苷的含量測定。
건립료궁염강과립중작약감적함량측정방법。채용반상고효액상색보법， YMC pack ODS-A C18색보주（150 mm ×4.6 mm，5μm）；을정-0.1％린산용액（12.5：87.5）위류동상；검측파장위230 nm，류속1.0 mL· min-1。결과표명：작약감재0.222～2.220μg범위내여봉면적정량호선성관계， r＝0.9999，작약감평균가양회수솔위99.19％， RSD위0.56％，정밀도、은정성량호。본방법가고성고，분리도、준학성、중복성호，괄용우궁염강과립중작약감적함량측정。
The HPLC method for determination of paeoniflorin in gongyankang granule was developed.The analytical column was YMC pack ODS-A C18 (4.6 mm ×150 mm, 5μm) filled with a 5μm stationary phase.The mobile phase was a mixture of acetonitrile -0.1%phodphric acid (12.5:87.5), and the flow-rate was 1 mL· min-1.The detector wavelength was set at 230 nm.The calibration curve was linear over the range of 0.222~2.220 μg for andrographolide ( r=0.9999 ) .The average recovery of paeoniflorin was 99.19%.The precision and stability were fine.The proposed method with high reliability, separation, accuracy, and repeatability may be used in the quantitative analysis of paeoniflorin in Gongyankang Granule.
