CAJ | 학술논문

采用CTAB法提取棕榈科植物基因组DNA，选用4个棕榈科植物DNA作模板，对100个SRAP引物组合进行筛选，以条带清晰多态性丰富为引物筛选原则，最后共筛选出14个组合作为棕榈科植物SRAP分子标记的核心引物，为后续SRAP分析提供依据。同时还对SRAP-PCR扩增程序进行优化，在不影响扩增效果的前提下，节约了大量实验时间。
채용CTAB법제취종려과식물기인조DNA，선용4개종려과식물DNA작모판，대100개SRAP인물조합진행사선，이조대청석다태성봉부위인물사선원칙，최후공사선출14개조합작위종려과식물SRAP분자표기적핵심인물，위후속SRAP분석제공의거。동시환대SRAP-PCR확증정서진행우화，재불영향확증효과적전제하，절약료대량실험시간。
The genomic DNAs were extracted from palm plants by using CTAB method , and 100 pairs of SRAP primers were screened for clear bands and rich polymorphism by using the extracted genomic DNAs from four palm plants as templates .Finally, 14 pairs of SRAP primers were screened out and were used as the core primers of palm plants SRAP molecular markers , which pro-vided the basis for the subsequent SRAP analysis in palm plants .This study also optimized the amplification program of SRAP -PCR, which could save much experimental time on the premise of not affecting the amplification effect .