CAJ | 학술논문

建立了采用超高效液相色谱-串联质谱法同时测定水中32种药物的方法，即：水样经稀硫酸酸化至pH 2．0，上样体积为500 mL，经HLB固相萃取小柱富集净化，超高效液相色谱-串联质谱仪测定， ESI正负离子同时扫描，以多反应监测（ MRM）方式采集数据，外标法定量。各药物的平均加标回收率为61．4％～116．0％，RSD为0．7％～17．3％，检出限为0．4～2．9 ng/L。
건립료채용초고효액상색보-천련질보법동시측정수중32충약물적방법，즉：수양경희류산산화지pH 2．0，상양체적위500 mL，경HLB고상췌취소주부집정화，초고효액상색보-천련질보의측정， ESI정부리자동시소묘，이다반응감측（ MRM）방식채집수거，외표법정량。각약물적평균가표회수솔위61．4％～116．0％，RSD위0．7％～17．3％，검출한위0．4～2．9 ng/L。
An ultra-high performance liquid chromatography -electrospray ionization tandem mass spectrometry ( UPLC-ESI-MS/MS) method was developed for the simultaneous determination of 32 drugs in water body .Firstly, the water sample was a-cidized to pH 2.0 by dilute sulphuric acid, and its volume was 500 mL;then the sample was richened and purified through solid -phase extraction with an Oasis HLB column;finally it was detected by using UPLC -ESI-MS/MS method.The ESI-MS/MS de-tection was achieved through the simultaneous scanning of ESI positive and negative ions , multiple reaction monitoring ( MRM) for data collection , and external standard method for quantitative analysis .The average standard -adding recovery of various drugs was 61.4%~116.0%, the RSD varied from 0.7%to 17.3%, and their detection limit was 0.4~2.9 ng/L.