化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2014年
7期
1117-1121
,共5页
磁性纳米Fe3 O4 粒子%制备%胰蛋白酶%活性
磁性納米Fe3 O4 粒子%製備%胰蛋白酶%活性
자성납미Fe3 O4 입자%제비%이단백매%활성
magnetic Fe3 O4 nanoparticles%preparation%trypsin%activity
采用微波法得到磁性纳米Fe304粒子,利用XRD,TEM等方法对其粒径大小及形貌进行了表征,结果分析表明产物为纳米级,粒径大小均匀。利用紫外可见吸收光谱及荧光光谱等方法研究了磁性纳米Fe304粒子与胰蛋白酶的相互作用,探讨了纳米粒子对胰蛋白酶的结构及活性的影响。
採用微波法得到磁性納米Fe304粒子,利用XRD,TEM等方法對其粒徑大小及形貌進行瞭錶徵,結果分析錶明產物為納米級,粒徑大小均勻。利用紫外可見吸收光譜及熒光光譜等方法研究瞭磁性納米Fe304粒子與胰蛋白酶的相互作用,探討瞭納米粒子對胰蛋白酶的結構及活性的影響。
채용미파법득도자성납미Fe304입자,이용XRD,TEM등방법대기립경대소급형모진행료표정,결과분석표명산물위납미급,립경대소균균。이용자외가견흡수광보급형광광보등방법연구료자성납미Fe304입자여이단백매적상호작용,탐토료납미입자대이단백매적결구급활성적영향。
The magnetic Fe3 O4 nanoparticles were prepared by microwave method. The structure and particle size were measured by XRD and TEM. The results showed that these nanometer particles have uniform size. The interactions between magnetic Fe3 O4 nano-particles and trypsin were investigated using UV-Vis and fluorescence spectral methods. The results showed that the process of bind-ing trypsin on Fe3 O4 nanoparticles was a spontaneous interaction procedure. The effects of nanoparticles on the conformation and ac-tivity of trypsin were also analyzed.
