CAJ | 학술논문

以乳房炎源性金黄色葡萄球菌分离株N2为研究对象，采用纸片扩散法分析了耐药性，应用刚果红法检测了生物被膜形成能力，利用PCR法扩增了8种生物被膜形成基因和9种肠毒素基因。结果表明，金黄色葡萄球菌分离株N2具有广泛的耐药性，仅对苯唑西林高度敏感，具有较强的生物被膜形成能力，携带除bap外所有的被测生物被膜相关基因和sea、sec、seg、sei 4种肠毒素基因。结果表示金黄色葡萄球菌分离株N2可能存在多种侵袭方式，在临床上难以防治，为进一步以该菌株为研究材料进行相关研究奠定了基础。
이유방염원성금황색포도구균분리주N2위연구대상，채용지편확산법분석료내약성，응용강과홍법검측료생물피막형성능력，이용PCR법확증료8충생물피막형성기인화9충장독소기인。결과표명，금황색포도구균분리주N2구유엄범적내약성，부대분서서림고도민감，구유교강적생물피막형성능력，휴대제bap외소유적피측생물피막상관기인화sea、sec、seg、sei 4충장독소기인。결과표시금황색포도구균분리주N2가능존재다충침습방식，재림상상난이방치，위진일보이해균주위연구재료진행상관연구전정료기출。
In this study, Staphylococcus aureus strain N2 was isolated from bovine subclinical mastitis , and its drug-resistance was analyzed by using disk diffusion method .The biofilm-forming capacity of N2 was detected by Congo Red Agar (CRA), and eight biofilm-associated genes and nine staphylococcal enterotoxin genes were amplified by using PCR method .The results showed that N2 had wide resistance to drugs , and it was highly sensitive only to Oxacillin .N2 had stronger ability to produce biofilm , and carried all detected biofilm -associated genes ( except for bap) and 4 enterotoxin genes ( sea, sec, seg, sei) .These results suggest that there probably are several invasive means into mammary epithelia tissue for N 2 , so it is difficult to prevent and control this strain.This study can lay the foundation for the further researches of Staphylococcus aureus strain N2 .