CAJ | 학술논문

目的：探讨苦参碱体外促进肿瘤细胞凋亡及其作用机制。方法将浓度为0：．2 mg/ml、0．4 mg/ml、0．6 mg/ml的苦参碱作用于卵巢癌细胞株A21-2和骨肉瘤细胞株MG63，利用倒置相差显微镜观察不同浓度苦参碱作用后细胞的形态变化；流式细胞术（FCM）检测细胞凋亡周期、Caspase-3蛋白的表达。结果倒置相差显微镜观察表明，苦参碱能抑制MG63细胞和A21-2细胞的增殖；FCM分析发现，苦参碱与细胞作用72h后，G0/G1期前出现凋亡峰，与对照组比较，观察组不同浓度下凋亡率升高（ P ＜0．01），且随浓度升高，凋亡率亦增高，差异有统计学意义（P ＜0．05），FCM分析显示，与对照组比较，观察组不同浓度下Caspase-3蛋白表达升高（ P ＜0．01），且随浓度升高， Caspase-3蛋白表达亦升高，差异有统计学意义（ P ＜0．05）。结论苦参碱能明显抑制A21-2细胞和MG63细胞的增殖，诱导细胞凋亡，其机制可能与促进Caspase-3蛋白的表达有关。
목적：탐토고삼감체외촉진종류세포조망급기작용궤제。방법장농도위0：．2 mg/ml、0．4 mg/ml、0．6 mg/ml적고삼감작용우란소암세포주A21-2화골육류세포주MG63，이용도치상차현미경관찰불동농도고삼감작용후세포적형태변화；류식세포술（FCM）검측세포조망주기、Caspase-3단백적표체。결과도치상차현미경관찰표명，고삼감능억제MG63세포화A21-2세포적증식；FCM분석발현，고삼감여세포작용72h후，G0/G1기전출현조망봉，여대조조비교，관찰조불동농도하조망솔승고（ P ＜0．01），차수농도승고，조망솔역증고，차이유통계학의의（P ＜0．05），FCM분석현시，여대조조비교，관찰조불동농도하Caspase-3단백표체승고（ P ＜0．01），차수농도승고， Caspase-3단백표체역승고，차이유통계학의의（ P ＜0．05）。결론고삼감능명현억제A21-2세포화MG63세포적증식，유도세포조망，기궤제가능여촉진Caspase-3단백적표체유관。
Objective To investigate the effect of matrine on induction of tumor cells apoptosis and its molecular mechanisms.Methods A21-2 and MG63 cells were treated with matrine at different concentration:0.2 mg/ml,0.4 mg/ml, 0.6 mg/ml.Using light microscope to observe the change of morphology;flow cytometry (FMC)method was used to detect the apoptosis cycles of cells and expression of Caspase-3 protein.Results After matrine treatment , inverted microscope observa-tion showed that the growth of cells was inhibited significantly .Flow cytometry method showed that there was a sub-G0/G1 apo-ptosis peak in the graph of drug-treated groups after 72h.Compared with control group , the apoptosis rate increased in obser-vation group( P <0.01),and the more the concentration was ,the higher the apoptosis rate was ( P <0.01).FCM analysis showed Caspase-3 increased in observation group than control group ( P <0.01),and alone with the increase of the concentra-tion,the expression of Caspase-3 increased( P <0.05).Conclusion Matrine can inhibit proliferation of A21-2 and MG63 cells.It's molecular mechanism might be related to modulating the expression of Caspase-3 protein.