CAJ | 학술논문

[目的]通过应用微流体芯片（ lab-on-chip）技术检测食源性致病菌，包括霍乱弧菌、沙门菌、副溶血性弧菌、志贺菌等细菌的灵敏度和精确度，从而建立突发公共卫生事件现场实验室的解决方案。[方法]分别采用Lab-on-chip平台食源性病原体微流检测芯片和荧光PCR方法对食源性病原菌样本进行检测，对比分析食源性微流芯片检测方法的特异性、敏感度、准确性和重复性。根据Lab-on-chip说明书以及荧光定量PCP试剂盒操作说明进行操作。[结果] Lab-on-chip方法的霍乱弧菌、沙门菌、志贺菌、副溶血性弧菌检出限为5×103～103 DNA Copies/mL。特异性、准确性和重复性都获得了良好的结果。[结论] Lab-on-chip方法具有快速、便捷、高效、准确等优点，可以在突发公共卫生事件中作为良好的检测工具和方法。
[목적]통과응용미류체심편（ lab-on-chip）기술검측식원성치병균，포괄곽란호균、사문균、부용혈성호균、지하균등세균적령민도화정학도，종이건립돌발공공위생사건현장실험실적해결방안。[방법]분별채용Lab-on-chip평태식원성병원체미류검측심편화형광PCR방법대식원성병원균양본진행검측，대비분석식원성미류심편검측방법적특이성、민감도、준학성화중복성。근거Lab-on-chip설명서이급형광정량PCP시제합조작설명진행조작。[결과] Lab-on-chip방법적곽란호균、사문균、지하균、부용혈성호균검출한위5×103～103 DNA Copies/mL。특이성、준학성화중복성도획득료량호적결과。[결론] Lab-on-chip방법구유쾌속、편첩、고효、준학등우점，가이재돌발공공위생사건중작위량호적검측공구화방법。
Objective To set up an onsite protocol for abrupt emergencies of public health e-vents, with both the sensitivity and accuracy of the lab-on-chip(microfluidic chip), for the detection of food borne pathogenic bacterium including Vibrio cholera , Salmonella , Vibrio parahaemolyticus and Shigellaare exanimated . [ Methods] By comparison of the results from the chips and fluorescence quantitative PCR were analyzed the specificity , sensitivity, accuracy and repeatability of the Lab-on-chip. [ Results] Acceptable specificity , accuracy and repeatability of the chips had been well achieved , the detection limit of the chips for the Vibrio cholera , Salmonella, Vibrio parahaemolyticus and Shigella was 5 ×103 ~103 DNA Copies/mL. [ Conclusion] Lab-on-chip is believed to be a fast , convenient , efficient tool with accept-able accuracy for public health emergencies .