特产研究
特產研究
특산연구
SPECIAL WILD ECONOMIC ANIMAL AND PLANT RESEARCH
2014年
2期
6-8,28
,共4页
仝明薇%易立%程世鹏
仝明薇%易立%程世鵬
동명미%역립%정세붕
细小病毒%实时荧光定量聚合酶链式反应%相对定量%β-肌动蛋白
細小病毒%實時熒光定量聚閤酶鏈式反應%相對定量%β-肌動蛋白
세소병독%실시형광정량취합매련식반응%상대정량%β-기동단백
parvovirus%real-time RT-PCR%relative quantification%β-actin
参考GenBank中已登录的CPV-SC-JM VP2基因序列设计引物，选用β-actin作为内参基因，利用SYBR Green Ⅰ实时定量PCR相对定量法，对规模化毛皮动物场的12份粪便样品和5份血液样品进行检测。常规PCR方法检出14份，检出率82％，荧光定量PCR相对定量方法检出17份，检出率100％；粪便中病毒含量明显高于血液中病毒含量，最高可达7000倍；不同毛皮动物粪便样品中病毒含量也存在较大差异。
參攷GenBank中已登錄的CPV-SC-JM VP2基因序列設計引物，選用β-actin作為內參基因，利用SYBR Green Ⅰ實時定量PCR相對定量法，對規模化毛皮動物場的12份糞便樣品和5份血液樣品進行檢測。常規PCR方法檢齣14份，檢齣率82％，熒光定量PCR相對定量方法檢齣17份，檢齣率100％；糞便中病毒含量明顯高于血液中病毒含量，最高可達7000倍；不同毛皮動物糞便樣品中病毒含量也存在較大差異。
삼고GenBank중이등록적CPV-SC-JM VP2기인서렬설계인물，선용β-actin작위내삼기인，이용SYBR Green Ⅰ실시정량PCR상대정량법，대규모화모피동물장적12빈분편양품화5빈혈액양품진행검측。상규PCR방법검출14빈，검출솔82％，형광정량PCR상대정량방법검출17빈，검출솔100％；분편중병독함량명현고우혈액중병독함량，최고가체7000배；불동모피동물분편양품중병독함량야존재교대차이。
A SYBR Green Ⅰ relative quantification real-time PCR method was used for detection of parvovirus loads in blood and faeces of using primers targeting the nuecleocide sequence logged in the GenBank .The results showed that the real -time PCR was more sensitive than the conventional PCR respectively with 100% and 82% positive detection rates .The levels of virus loads in faeces were significantly higher than that in the blood ,up to 7000-fold ,it also various largely in different faeces samples from diverse fur animals .
