特产研究
特產研究
특산연구
SPECIAL WILD ECONOMIC ANIMAL AND PLANT RESEARCH
2014年
2期
1-5
,共5页
张海玲%张蕾%胡博%白雪%赵建军%刘昊%徐淑娟%苗立光%冯卓%闫喜军
張海玲%張蕾%鬍博%白雪%趙建軍%劉昊%徐淑娟%苗立光%馮卓%閆喜軍
장해령%장뢰%호박%백설%조건군%류호%서숙연%묘립광%풍탁%염희군
水貂肠炎病毒%NS1基因%分子流行病学调查
水貂腸炎病毒%NS1基因%分子流行病學調查
수초장염병독%NS1기인%분자류행병학조사
mink enteritis virus%NS1 gene%molecular epidemiologic investigation introduction
采用PCR方法，从辽宁省、吉林省、山东省、河北省采集的32份样品中检测出21份水貂肠炎病毒感染阳性样品。每个地区选取有代表性的样品共计11份进行NS1基因的克隆和测序，并与已知参考毒株进行比对及系统发育分析。结果显示，本次检测的11个阳性样品的核苷酸序列和氨基酸序列同源性为98.8％～100.0％和98.5％～100％；本次测序序列和国内毒株MEVB基因的核苷酸和氨基酸序列同源性为98.7％～99.9％和98.7％～99.9％；与国外毒株MEVAbashiri基因的核苷酸和氨基酸序列同源性为99.0％～99.5％和98.7％～99.1％。11份阳性样品中水貂肠炎病毒的NS1基因有24个氨基酸位点存在变异。11份阳性样品可划分为3个分支。
採用PCR方法，從遼寧省、吉林省、山東省、河北省採集的32份樣品中檢測齣21份水貂腸炎病毒感染暘性樣品。每箇地區選取有代錶性的樣品共計11份進行NS1基因的剋隆和測序，併與已知參攷毒株進行比對及繫統髮育分析。結果顯示，本次檢測的11箇暘性樣品的覈苷痠序列和氨基痠序列同源性為98.8％～100.0％和98.5％～100％；本次測序序列和國內毒株MEVB基因的覈苷痠和氨基痠序列同源性為98.7％～99.9％和98.7％～99.9％；與國外毒株MEVAbashiri基因的覈苷痠和氨基痠序列同源性為99.0％～99.5％和98.7％～99.1％。11份暘性樣品中水貂腸炎病毒的NS1基因有24箇氨基痠位點存在變異。11份暘性樣品可劃分為3箇分支。
채용PCR방법，종요녕성、길림성、산동성、하북성채집적32빈양품중검측출21빈수초장염병독감염양성양품。매개지구선취유대표성적양품공계11빈진행NS1기인적극륭화측서，병여이지삼고독주진행비대급계통발육분석。결과현시，본차검측적11개양성양품적핵감산서렬화안기산서렬동원성위98.8％～100.0％화98.5％～100％；본차측서서렬화국내독주MEVB기인적핵감산화안기산서렬동원성위98.7％～99.9％화98.7％～99.9％；여국외독주MEVAbashiri기인적핵감산화안기산서렬동원성위99.0％～99.5％화98.7％～99.1％。11빈양성양품중수초장염병독적NS1기인유24개안기산위점존재변이。11빈양성양품가화분위3개분지。
Twenty-one of 32 tissue samples from the diseased minks in Liaoning ,Jilin ,Shandong and Hebei Provinces were tested positive for mink enteritis virus(MEV) .The NS1 genes from the 11 selected positive samples were cloned for sequencing respectively .Alignment and phylo-genetic analysis were done with sequences from known reference strains .The results showed that the NS1 genes between the 11 representative positive samples shared 98.8% -100.0% identity at nucleotide level and 98.5% -100% at amino acid leve1 .The homology between the 11 positive samples and the MEVB strains in China ranged from 98.7% -99.9% and 98.7% -99.9% at NS1 gene nucleotide sequence and the deduced amino acid sequence ,respectively .The homology among the 11 samples and the foreign strains ranged from 99.0% -99.5% , 98.7% -99.1% at NS1 gene's nucleotide sequence .Twenty four mutant amino acid sites from the NS1 gene were found .The 11 positive sam-ples could be divided into three clades .
