CAJ | 학술논문

目的：研究中药单体蛇床子素( Osthole, Ost)与5-FU ( Fluorouracil,5-FU)在人胃癌细胞株BGC-823以及人大肠癌细胞株LOVO中的体外相互作用。方法采用MTT法检测单药及联合用药对两株肿瘤细胞株的作用；利用中效原理评价两药之间的相互作用；利用流式细胞仪检测单药及联合用药对肿瘤细胞凋亡和周期的影响。结果Ost和5-FU对两株肿瘤细胞株均具有增殖抑制作用；作用于BGC-823细胞时,当Fa<0．65时, CI<1,两药合用效应为协同作用；作用于LOVO细胞时,当0．05<Fa<0．90时, CI<1,两药合用效应为协同作用。结论 Ost联合5-FU作用于BGC-823细胞在低剂量合用时产生协同效应,协同机制可能与诱导细胞凋亡及G2/M期阻滞相关。
목적：연구중약단체사상자소( Osthole, Ost)여5-FU ( Fluorouracil,5-FU)재인위암세포주BGC-823이급인대장암세포주LOVO중적체외상호작용。방법채용MTT법검측단약급연합용약대량주종류세포주적작용；이용중효원리평개량약지간적상호작용；이용류식세포의검측단약급연합용약대종류세포조망화주기적영향。결과Ost화5-FU대량주종류세포주균구유증식억제작용；작용우BGC-823세포시,당Fa<0．65시, CI<1,량약합용효응위협동작용；작용우LOVO세포시,당0．05<Fa<0．90시, CI<1,량약합용효응위협동작용。결론 Ost연합5-FU작용우BGC-823세포재저제량합용시산생협동효응,협동궤제가능여유도세포조망급G2/M기조체상관。
Objective To study the in vitro interactions between osthole ( ost) and fluorouracil ( 5-FU) on human gastric carcinoma cell lines BGC-823 and the human colon cancer cell lines LOVO. Method For single agent and com-bined medication, the effects on two tumor cell lines, the interactions, and the influences on tumor cell cycle and apopto-sis were analyzed using MTT method, median-effect principle and flow cytometry, respectively. Result The combination of ost and 5-FU inhibited the proliferation of the two tumor cell lines; For BGC-823 cells, when Fa<0. 65, CI<1, the combined effects of the two medicine were synergies;For LOVO cells, when 0. 05<Fa<0. 90, CI<1, the effects were also synergies. Conclusion The combined effects of ost and 5-FU on BGC-823 cells were synergistic when in low doses, and the mechanism may be related to apoptosis induced and the block of G2/M phase.