广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2014年
18期
149-151
,共3页
全自动固相萃取%超高压液相色谱( UPLC)%合成色素%食品
全自動固相萃取%超高壓液相色譜( UPLC)%閤成色素%食品
전자동고상췌취%초고압액상색보( UPLC)%합성색소%식품
automatic solid phase extraction%UPLC%synthetic colorants%food
建立了全自动固相萃取/超高压液相色谱法同时测定食品中10种人工合成色素柠檬黄、苋菜红、胭脂红、日落黄、亮蓝、诱惑红、靛蓝、赤藓红、新红、酸性红的方法。液体样品经加热驱除二氧化碳和酒精，固体或半固体样品经乙醇-水-氨水（70＋29＋1）溶液提取，所得待净化液采用全自动固相萃取仪经Strata X-AW SPE小柱净化，采用超高压液相色谱进行分析。用甲醇和0.02 mol/L乙酸铵溶液作为流动相进行梯度洗脱，流速0.3 mL/min，在4 min内实现10种合成色素的良好分离。10种色素在1～50μg/mL的范围内线性良好。10种色素的方法最低检出限是0.004 mg/kg，加标回收率在83.8％～111.2％之间，相对标准偏差在1.24％～4.18％之间（n＝6）。本方法操作简单，灵敏度高，干扰少，能满足食品中多种合成色素同时分析测定要求。
建立瞭全自動固相萃取/超高壓液相色譜法同時測定食品中10種人工閤成色素檸檬黃、莧菜紅、胭脂紅、日落黃、亮藍、誘惑紅、靛藍、赤蘚紅、新紅、痠性紅的方法。液體樣品經加熱驅除二氧化碳和酒精，固體或半固體樣品經乙醇-水-氨水（70＋29＋1）溶液提取，所得待淨化液採用全自動固相萃取儀經Strata X-AW SPE小柱淨化，採用超高壓液相色譜進行分析。用甲醇和0.02 mol/L乙痠銨溶液作為流動相進行梯度洗脫，流速0.3 mL/min，在4 min內實現10種閤成色素的良好分離。10種色素在1～50μg/mL的範圍內線性良好。10種色素的方法最低檢齣限是0.004 mg/kg，加標迴收率在83.8％～111.2％之間，相對標準偏差在1.24％～4.18％之間（n＝6）。本方法操作簡單，靈敏度高，榦擾少，能滿足食品中多種閤成色素同時分析測定要求。
건립료전자동고상췌취/초고압액상색보법동시측정식품중10충인공합성색소저몽황、현채홍、연지홍、일락황、량람、유혹홍、전람、적선홍、신홍、산성홍적방법。액체양품경가열구제이양화탄화주정，고체혹반고체양품경을순-수-안수（70＋29＋1）용액제취，소득대정화액채용전자동고상췌취의경Strata X-AW SPE소주정화，채용초고압액상색보진행분석。용갑순화0.02 mol/L을산안용액작위류동상진행제도세탈，류속0.3 mL/min，재4 min내실현10충합성색소적량호분리。10충색소재1～50μg/mL적범위내선성량호。10충색소적방법최저검출한시0.004 mg/kg，가표회수솔재83.8％～111.2％지간，상대표준편차재1.24％～4.18％지간（n＝6）。본방법조작간단，령민도고，간우소，능만족식품중다충합성색소동시분석측정요구。
An automatic solid phase extraction/ultra high pressure liquid chromatographic method was established for the simultaneous determination of 10 synthetic colorants tartrazine, amaranth, ponceau 4R, sunset yellow, brilliant blue, allura red, indigotine, erythrosine, new red, and carmoisine in food.Liquid samples were removed carbon dioxide and alcohol by heating , solid or semi-solid samples were extracted with alcohol -water-ammonia ( 70+29+1 ) solution , and the extract was purified on Strata X -AW SPE column by automatic solid phase extraction apparatus , than analyzed by UPLC.The mobile phase was methanol and 0.02 mol/L ammonium acetate solution in gradient condition.The flow rate was 0.3 mL/min.Using the best gradient condition , ten synthetic colorants achieved the grate separation in 4 min.All the ten synthetic colorants had a good linearity in a concentration range of 1 ~50 μg/mL.The detect limit of the method was 0.004 mg/kg.The spiked recoveries of ten colorants ranged from 83.8% to 111.2%, with the RSDs of 1.24%~4.18%( n =6 ).This method was simple , sensitive and anti -interfere , which can be used for the determination of multiple synthetic colorants in food.
