中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
27期
9-11,25
,共4页
迟淑萍%张诗龙%陈红鸽%刘军%杜丽%孙杰%蒋正杰%程云
遲淑萍%張詩龍%陳紅鴿%劉軍%杜麗%孫傑%蔣正傑%程雲
지숙평%장시룡%진홍합%류군%두려%손걸%장정걸%정운
S180%蛋白免疫印迹法%P53%P21%Bcl-2
S180%蛋白免疫印跡法%P53%P21%Bcl-2
S180%단백면역인적법%P53%P21%Bcl-2
S180%Western blotting%P53%P21%Bcl-2
目的:通过动物实验,观察S180瘤株对小鼠荷瘤形成的影响,探讨S180肉瘤细胞的致瘤机制。方法建立小鼠实体瘤模型,将昆明系小鼠36只,按体重随机分为3组,每组12只。空白组:每日灌胃给予蒸馏水,0.2 mL/10 g体重;模型组:皮下接种S180肉瘤细胞,制成小鼠实体瘤模型,其后每日口服灌胃1%羧甲基纤维素钠(CMC)溶液;环磷酰胺组:皮下接种S180肉瘤细胞,制成小鼠实体瘤模型后,注射0.02 g/(kg·d)环磷酰胺注射液。观察各组肿瘤生长情况,计算平均重量;与模型组比较,环磷酰胺组计算肿瘤生长抑制率;采用蛋白免疫印迹法技术方法,检测抑癌基因P53、P21及凋亡抑制基因Bcl-2在S180肿瘤动物模型瘤体组织中的蛋白表达。结果模型组小鼠荷瘤成功,与空白组比较,模型组小鼠出现肿瘤,其瘤重为(1.513±0.790)g,环磷酰胺组瘤重为(0.248±0.253)g,两组比较差异有高度统计学意义(P<0.01),环磷酰胺抑瘤率为83.63%。在被检瘤体中,三种蛋白表达空白组与模型组、空白组与环磷酰胺组相比差异均有高度统计学意义(P<0.01);与环磷酰胺组比较,模型组P53、P21抗体的蛋白表达量明显降低,差异有统计学意义(P<0.05),其中,P21表达差异有高度统计学意义(P<0.01),而Bcl-2变化不大,差异无统计学意义(P=0.66)。结论 S180肉瘤细胞可能通过抑制P53与P21的表达而起到促进肿瘤形成的作用,该机制的研究可为筛选肿瘤治疗药物及肿瘤机制探讨等的肿瘤动物模型提供重要的实验±据。
目的:通過動物實驗,觀察S180瘤株對小鼠荷瘤形成的影響,探討S180肉瘤細胞的緻瘤機製。方法建立小鼠實體瘤模型,將昆明繫小鼠36隻,按體重隨機分為3組,每組12隻。空白組:每日灌胃給予蒸餾水,0.2 mL/10 g體重;模型組:皮下接種S180肉瘤細胞,製成小鼠實體瘤模型,其後每日口服灌胃1%羧甲基纖維素鈉(CMC)溶液;環燐酰胺組:皮下接種S180肉瘤細胞,製成小鼠實體瘤模型後,註射0.02 g/(kg·d)環燐酰胺註射液。觀察各組腫瘤生長情況,計算平均重量;與模型組比較,環燐酰胺組計算腫瘤生長抑製率;採用蛋白免疫印跡法技術方法,檢測抑癌基因P53、P21及凋亡抑製基因Bcl-2在S180腫瘤動物模型瘤體組織中的蛋白錶達。結果模型組小鼠荷瘤成功,與空白組比較,模型組小鼠齣現腫瘤,其瘤重為(1.513±0.790)g,環燐酰胺組瘤重為(0.248±0.253)g,兩組比較差異有高度統計學意義(P<0.01),環燐酰胺抑瘤率為83.63%。在被檢瘤體中,三種蛋白錶達空白組與模型組、空白組與環燐酰胺組相比差異均有高度統計學意義(P<0.01);與環燐酰胺組比較,模型組P53、P21抗體的蛋白錶達量明顯降低,差異有統計學意義(P<0.05),其中,P21錶達差異有高度統計學意義(P<0.01),而Bcl-2變化不大,差異無統計學意義(P=0.66)。結論 S180肉瘤細胞可能通過抑製P53與P21的錶達而起到促進腫瘤形成的作用,該機製的研究可為篩選腫瘤治療藥物及腫瘤機製探討等的腫瘤動物模型提供重要的實驗±據。
목적:통과동물실험,관찰S180류주대소서하류형성적영향,탐토S180육류세포적치류궤제。방법건립소서실체류모형,장곤명계소서36지,안체중수궤분위3조,매조12지。공백조:매일관위급여증류수,0.2 mL/10 g체중;모형조:피하접충S180육류세포,제성소서실체류모형,기후매일구복관위1%최갑기섬유소납(CMC)용액;배린선알조:피하접충S180육류세포,제성소서실체류모형후,주사0.02 g/(kg·d)배린선알주사액。관찰각조종류생장정황,계산평균중량;여모형조비교,배린선알조계산종류생장억제솔;채용단백면역인적법기술방법,검측억암기인P53、P21급조망억제기인Bcl-2재S180종류동물모형류체조직중적단백표체。결과모형조소서하류성공,여공백조비교,모형조소서출현종류,기류중위(1.513±0.790)g,배린선알조류중위(0.248±0.253)g,량조비교차이유고도통계학의의(P<0.01),배린선알억류솔위83.63%。재피검류체중,삼충단백표체공백조여모형조、공백조여배린선알조상비차이균유고도통계학의의(P<0.01);여배린선알조비교,모형조P53、P21항체적단백표체량명현강저,차이유통계학의의(P<0.05),기중,P21표체차이유고도통계학의의(P<0.01),이Bcl-2변화불대,차이무통계학의의(P=0.66)。결론 S180육류세포가능통과억제P53여P21적표체이기도촉진종류형성적작용,해궤제적연구가위사선종류치료약물급종류궤제탐토등적종류동물모형제공중요적실험±거。
Objective To investigate the tumorigenic mechanism of sarcoma cell line (S180) through observing the ef-fects of S180 on bearing tumor formation in mice. Methods Establishment of solid tumor model in mice, 60 Kunming mice were randomly divided into negative control group, model group and positive control group (Cyclophosphamide group) according to the body weight, 12 mice in each group. The negative control group was given distilled water, 0.2 mL/10 g. Except for the negative control group, subcutaneous injection with S180 sarcomas cells was done in the mice of other two groups. After the tumor model of mice was established, the model group mice were lavaged daily with 1% sodium carboxymethyl cellulose solution, and Cyclophosphamide group mice were injected Cyclophosphamide, 0.02 g/(kg·d). Then the tumor's volume and weight were measured, the tumor growth inhibition rate was calculated, and the expres-sion of tissue protein of three kinds of antibody including tumor suppressor gene (P53 and P21) and apoptosis inhibiting gene (Bcl-2) were detected by Western blotting technology. Results The S180 cells planted tumor model of mice was es-tablished but not appearanced in negative control group. The tumor weight in model group and Cyclophosphamide group was (1.513±0.790) g and (0.248±0.253) g, respectively, it was significantly different (P< 0.01) between the two groups. The inhibitory rate of positive control group was 83.63%. Compared with positive control group, the P53, P21 and Bcl-2 protein levels were significantly different in the model group and Cyclophosphamide group (P<0.01). Com-pared with positive control group, the P53 and P21 protein level significantly decreased in model group (P<0.05), the difference of P21 was highly statistically significant (P< 0.01), the changes of Bcl-2 were not statistically significant(P=0.66). Conclusion The S180 sarcoma cells may pro-mote tumor formation by inhibiting expression of P53 and P21. This mechanism can provide important experi-mental evidence for tumor animal model for screening drug and tumor mechanism.